Clock mutation affects circadian regulation of circulating blood cells

Katsutaka Oishi, Naoki Ohkura, Koji Kadota, Manami Kasamatsu, Kentaro Shibusawa, Juzo Matsuda, Kazuhiko Machida, Shuichi Horie, Norio Ishida

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Background: Although the number of circulating immune cells is subject to high-amplitude circadian rhythms, the underlying mechanisms are not fully understood. Methods: To determine whether intact CLOCK protein is required for the circadian changes in peripheral blood cells, we examined circulating white (WBC) and red (RBC) blood cells in homozygous Clock mutant mice. Results: Daytime increases in total WBC and lymphocytes were suppressed and slightly phase-delayed along with plasma corticosterone levels in Clock mutant mice. The peak RBC rhythm was significantly reduced and phase-advanced in the Clock mutants. Anatomical examination revealed hemoglobin-rich, swollen red spleens in Clock mutant mice, suggesting RBC accumulation. Conclusion: Our results suggest that endogenous clock-regulated circadian corticosterone secretion from the adrenal gland is involved in the effect of a Clock mutation on daily profiles of circulating WBC. However, intact CLOCK seems unnecessary for generating the rhythm of corticosterone secretion in mice. Our results also suggest that CLOCK is involved in discharge of RBC from the spleen.

Original languageEnglish
Article number13
JournalJournal of Circadian Rhythms
Volume4
DOIs
Publication statusPublished - 2006 Oct 2

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Blood Cells
Corticosterone
Mutation
CLOCK Proteins
Spleen
Circadian Clocks
Adrenal Glands
Circadian Rhythm
Hemoglobins
Erythrocytes
Lymphocytes

ASJC Scopus subject areas

  • Physiology

Cite this

Oishi, K., Ohkura, N., Kadota, K., Kasamatsu, M., Shibusawa, K., Matsuda, J., ... Ishida, N. (2006). Clock mutation affects circadian regulation of circulating blood cells. Journal of Circadian Rhythms, 4, [13]. https://doi.org/10.1186/1740-3391-4-13

Clock mutation affects circadian regulation of circulating blood cells. / Oishi, Katsutaka; Ohkura, Naoki; Kadota, Koji; Kasamatsu, Manami; Shibusawa, Kentaro; Matsuda, Juzo; Machida, Kazuhiko; Horie, Shuichi; Ishida, Norio.

In: Journal of Circadian Rhythms, Vol. 4, 13, 02.10.2006.

Research output: Contribution to journalArticle

Oishi, K, Ohkura, N, Kadota, K, Kasamatsu, M, Shibusawa, K, Matsuda, J, Machida, K, Horie, S & Ishida, N 2006, 'Clock mutation affects circadian regulation of circulating blood cells', Journal of Circadian Rhythms, vol. 4, 13. https://doi.org/10.1186/1740-3391-4-13
Oishi K, Ohkura N, Kadota K, Kasamatsu M, Shibusawa K, Matsuda J et al. Clock mutation affects circadian regulation of circulating blood cells. Journal of Circadian Rhythms. 2006 Oct 2;4. 13. https://doi.org/10.1186/1740-3391-4-13
Oishi, Katsutaka ; Ohkura, Naoki ; Kadota, Koji ; Kasamatsu, Manami ; Shibusawa, Kentaro ; Matsuda, Juzo ; Machida, Kazuhiko ; Horie, Shuichi ; Ishida, Norio. / Clock mutation affects circadian regulation of circulating blood cells. In: Journal of Circadian Rhythms. 2006 ; Vol. 4.
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AU - Matsuda, Juzo

AU - Machida, Kazuhiko

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