Cloning and characterization of mevalonate pathway genes in a natural rubber producing plant, hevea brasiliensis

Tomoki Sando, Chika Takaoka, Yukio Mukai, Atsushi Yamashita, Masahira Hattori, Naotake Ogasawara, Eiichiro Fukusaki, Akio Kobayashi

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Hevea brasiliensis Müll. Arg. is a tree that produces natural rubber, an industrially vital isoprenoid polymer. Biosynthesis of natural rubber is known to take place biochemically by a mevalonate (MVA) pathway, but molecular biological characterization of related genes has been insufficient. From H. brasiliensis, we obtained full-length cDNA of genes encoding all of the enzymes that catalyze the six steps of the MVA pathway. Alignment analysis and phylogenetic analysis revealed that in H. brasiliensis there are three acetyl-CoA acetyltransferase genes, two HMG-CoA synthase (HMGS) genes, and four HMG-CoA reductase (HMGR) genes. Gene expression analysis by type of tissue indicated that MVA pathway genes were highly expressed in latex, as compared to other types of tissue and that HMGS and HMGR, which exist in multiple copies, have different expression patterns. Moreover, these MVA pathway genes in H. brasiliensis were found to complement MVA pathway deletion mutations in yeast.

Original languageEnglish
Pages (from-to)2049-2060
Number of pages12
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number8
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Hevea
Mevalonic Acid
Hevea brasiliensis
Cloning
Rubber
rubber
Organism Cloning
molecular cloning
Genes
Hydroxymethylglutaryl-CoA Synthase
Hydroxymethylglutaryl CoA Reductases
genes
Acetyl-CoA C-Acetyltransferase
Tissue
acetyl-CoA acetyltransferase
Gene encoding
Biosynthesis
Terpenes
Latex
Latexes

Keywords

  • cis-polyisoprene
  • Hevea brasiliensis
  • Mevalonate (MVA) pathway
  • Natural rubber
  • Yeast complementation

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Cloning and characterization of mevalonate pathway genes in a natural rubber producing plant, hevea brasiliensis. / Sando, Tomoki; Takaoka, Chika; Mukai, Yukio; Yamashita, Atsushi; Hattori, Masahira; Ogasawara, Naotake; Fukusaki, Eiichiro; Kobayashi, Akio.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 8, 2008, p. 2049-2060.

Research output: Contribution to journalArticle

Sando, Tomoki ; Takaoka, Chika ; Mukai, Yukio ; Yamashita, Atsushi ; Hattori, Masahira ; Ogasawara, Naotake ; Fukusaki, Eiichiro ; Kobayashi, Akio. / Cloning and characterization of mevalonate pathway genes in a natural rubber producing plant, hevea brasiliensis. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 8. pp. 2049-2060.
@article{20779769d7214e7bab6d047d4fa836d5,
title = "Cloning and characterization of mevalonate pathway genes in a natural rubber producing plant, hevea brasiliensis",
abstract = "Hevea brasiliensis M{\"u}ll. Arg. is a tree that produces natural rubber, an industrially vital isoprenoid polymer. Biosynthesis of natural rubber is known to take place biochemically by a mevalonate (MVA) pathway, but molecular biological characterization of related genes has been insufficient. From H. brasiliensis, we obtained full-length cDNA of genes encoding all of the enzymes that catalyze the six steps of the MVA pathway. Alignment analysis and phylogenetic analysis revealed that in H. brasiliensis there are three acetyl-CoA acetyltransferase genes, two HMG-CoA synthase (HMGS) genes, and four HMG-CoA reductase (HMGR) genes. Gene expression analysis by type of tissue indicated that MVA pathway genes were highly expressed in latex, as compared to other types of tissue and that HMGS and HMGR, which exist in multiple copies, have different expression patterns. Moreover, these MVA pathway genes in H. brasiliensis were found to complement MVA pathway deletion mutations in yeast.",
keywords = "cis-polyisoprene, Hevea brasiliensis, Mevalonate (MVA) pathway, Natural rubber, Yeast complementation",
author = "Tomoki Sando and Chika Takaoka and Yukio Mukai and Atsushi Yamashita and Masahira Hattori and Naotake Ogasawara and Eiichiro Fukusaki and Akio Kobayashi",
year = "2008",
doi = "10.1271/bbb.80165",
language = "English",
volume = "72",
pages = "2049--2060",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "8",

}

TY - JOUR

T1 - Cloning and characterization of mevalonate pathway genes in a natural rubber producing plant, hevea brasiliensis

AU - Sando, Tomoki

AU - Takaoka, Chika

AU - Mukai, Yukio

AU - Yamashita, Atsushi

AU - Hattori, Masahira

AU - Ogasawara, Naotake

AU - Fukusaki, Eiichiro

AU - Kobayashi, Akio

PY - 2008

Y1 - 2008

N2 - Hevea brasiliensis Müll. Arg. is a tree that produces natural rubber, an industrially vital isoprenoid polymer. Biosynthesis of natural rubber is known to take place biochemically by a mevalonate (MVA) pathway, but molecular biological characterization of related genes has been insufficient. From H. brasiliensis, we obtained full-length cDNA of genes encoding all of the enzymes that catalyze the six steps of the MVA pathway. Alignment analysis and phylogenetic analysis revealed that in H. brasiliensis there are three acetyl-CoA acetyltransferase genes, two HMG-CoA synthase (HMGS) genes, and four HMG-CoA reductase (HMGR) genes. Gene expression analysis by type of tissue indicated that MVA pathway genes were highly expressed in latex, as compared to other types of tissue and that HMGS and HMGR, which exist in multiple copies, have different expression patterns. Moreover, these MVA pathway genes in H. brasiliensis were found to complement MVA pathway deletion mutations in yeast.

AB - Hevea brasiliensis Müll. Arg. is a tree that produces natural rubber, an industrially vital isoprenoid polymer. Biosynthesis of natural rubber is known to take place biochemically by a mevalonate (MVA) pathway, but molecular biological characterization of related genes has been insufficient. From H. brasiliensis, we obtained full-length cDNA of genes encoding all of the enzymes that catalyze the six steps of the MVA pathway. Alignment analysis and phylogenetic analysis revealed that in H. brasiliensis there are three acetyl-CoA acetyltransferase genes, two HMG-CoA synthase (HMGS) genes, and four HMG-CoA reductase (HMGR) genes. Gene expression analysis by type of tissue indicated that MVA pathway genes were highly expressed in latex, as compared to other types of tissue and that HMGS and HMGR, which exist in multiple copies, have different expression patterns. Moreover, these MVA pathway genes in H. brasiliensis were found to complement MVA pathway deletion mutations in yeast.

KW - cis-polyisoprene

KW - Hevea brasiliensis

KW - Mevalonate (MVA) pathway

KW - Natural rubber

KW - Yeast complementation

UR - http://www.scopus.com/inward/record.url?scp=50649109455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50649109455&partnerID=8YFLogxK

U2 - 10.1271/bbb.80165

DO - 10.1271/bbb.80165

M3 - Article

VL - 72

SP - 2049

EP - 2060

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 8

ER -