Cloning and characterization of the promoter of murine cytohesin-1 gene

Nobuhito Goda, Akito Tanoue, Shintaro Kikuchi, Gozoh Tsujimoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)195-199
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1493
Issue number1-2
DOIs
Publication statusPublished - 2000 Sep 7
Externally publishedYes

Keywords

  • Cytohesin-1
  • Murine
  • Promoter
  • Sp-1

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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