Cloning and characterization of the promoter of murine cytohesin-1 gene

Nobuhito Goda; Akito Tanoue; Shintaro Kikuchi; Gozoh Tsujimoto

doi:10.1016/S0167-4781(00)00160-3

Cloning and characterization of the promoter of murine cytohesin-1 gene

Nobuhito Goda, Akito Tanoue^*, Shintaro Kikuchi, Gozoh Tsujimoto

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	195-199
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1493
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(00)00160-3
Publication status	Published - 2000 Sept 7
Externally published	Yes

Keywords

Cytohesin-1
Murine
Promoter
Sp-1

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(00)00160-3

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title = "Cloning and characterization of the promoter of murine cytohesin-1 gene",

abstract = "Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.",

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author = "Nobuhito Goda and Akito Tanoue and Shintaro Kikuchi and Gozoh Tsujimoto",

note = "Funding Information: Nobuhito Goda is a research fellow supported by the Japan Society for the Promotion of Science in 1999. This work was supported in part by research grants from the Scientific Fund of the Ministry of Education, Science, and Culture of Japan, the Japan Health Science Foundation and Ministry of Human Health and Welfare, the Organization for Pharmaceutical Safety and Research (OPSR), and a Grant for Liberal Harmonious Research Promotion System from the Science and Technology Agency.",

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AU - Goda, Nobuhito

AU - Tanoue, Akito

AU - Kikuchi, Shintaro

AU - Tsujimoto, Gozoh

N1 - Funding Information: Nobuhito Goda is a research fellow supported by the Japan Society for the Promotion of Science in 1999. This work was supported in part by research grants from the Scientific Fund of the Ministry of Education, Science, and Culture of Japan, the Japan Health Science Foundation and Ministry of Human Health and Welfare, the Organization for Pharmaceutical Safety and Research (OPSR), and a Grant for Liberal Harmonious Research Promotion System from the Science and Technology Agency.

PY - 2000/9/7

Y1 - 2000/9/7

N2 - Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.

AB - Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.

KW - Cytohesin-1

KW - Murine

KW - Promoter

KW - Sp-1

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Cloning and characterization of the promoter of murine cytohesin-1 gene

Abstract

Keywords

ASJC Scopus subject areas

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