Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus

Hiroko Ikeshima-Kataoka, Adumi Wada, Kenji Ishiwata, Naohiro Watanabe, Saburo Saito

Research output: Contribution to journalArticle

Abstract

The 5′-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase chain reaction (PCR) methodology. The cDNA sequence of IL-4 of MSKR was found to contain several nucleotide alterations, which result in amino acid substitutions, in comparison with that of inbred mouse BALB/c. In MSKR, IL-4 was expressed at high levels in thymus and spleen as revealed by reverse transcriptase PCR method and Northern hybridization. The tissue-specific expression profile was quite similar to that of laboratory mice.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalIn Vivo
Volume23
Issue number2
Publication statusPublished - 2009 Mar
Externally publishedYes

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Keywords

  • 5′-upstream region
  • cDNA
  • Interleukin 4(IL-4)Thymus
  • MSKR inbred strain of Mus musculus molossinus
  • Reverse transcriptase-polymerase chain reaction (RT-PCR)
  • Spleen

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Ikeshima-Kataoka, H., Wada, A., Ishiwata, K., Watanabe, N., & Saito, S. (2009). Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus. In Vivo, 23(2), 277-280.