Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus

Hiroko Ikeshima-Kataoka; Adumi Wada; Kenji Ishiwata; Naohiro Watanabe; Saburo Saito

Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus

Hiroko Ikeshima-Kataoka^*, Adumi Wada, Kenji Ishiwata, Naohiro Watanabe, Saburo Saito

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The 5′-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase chain reaction (PCR) methodology. The cDNA sequence of IL-4 of MSKR was found to contain several nucleotide alterations, which result in amino acid substitutions, in comparison with that of inbred mouse BALB/c. In MSKR, IL-4 was expressed at high levels in thymus and spleen as revealed by reverse transcriptase PCR method and Northern hybridization. The tissue-specific expression profile was quite similar to that of laboratory mice.

Original language	English
Pages (from-to)	277-280
Number of pages	4
Journal	In Vivo
Volume	23
Issue number	2
Publication status	Published - 2009 Mar
Externally published	Yes

Keywords

5′-upstream region
cDNA
Interleukin 4(IL-4)Thymus
MSKR inbred strain of Mus musculus molossinus
Reverse transcriptase-polymerase chain reaction (RT-PCR)
Spleen

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology

Cite this

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title = "Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus",

abstract = "The 5′-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase chain reaction (PCR) methodology. The cDNA sequence of IL-4 of MSKR was found to contain several nucleotide alterations, which result in amino acid substitutions, in comparison with that of inbred mouse BALB/c. In MSKR, IL-4 was expressed at high levels in thymus and spleen as revealed by reverse transcriptase PCR method and Northern hybridization. The tissue-specific expression profile was quite similar to that of laboratory mice.",

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T1 - Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus

AU - Ikeshima-Kataoka, Hiroko

AU - Wada, Adumi

AU - Ishiwata, Kenji

AU - Watanabe, Naohiro

AU - Saito, Saburo

PY - 2009/3

Y1 - 2009/3

N2 - The 5′-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase chain reaction (PCR) methodology. The cDNA sequence of IL-4 of MSKR was found to contain several nucleotide alterations, which result in amino acid substitutions, in comparison with that of inbred mouse BALB/c. In MSKR, IL-4 was expressed at high levels in thymus and spleen as revealed by reverse transcriptase PCR method and Northern hybridization. The tissue-specific expression profile was quite similar to that of laboratory mice.

AB - The 5′-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase chain reaction (PCR) methodology. The cDNA sequence of IL-4 of MSKR was found to contain several nucleotide alterations, which result in amino acid substitutions, in comparison with that of inbred mouse BALB/c. In MSKR, IL-4 was expressed at high levels in thymus and spleen as revealed by reverse transcriptase PCR method and Northern hybridization. The tissue-specific expression profile was quite similar to that of laboratory mice.

KW - 5′-upstream region

KW - cDNA

KW - Interleukin 4(IL-4)Thymus

KW - MSKR inbred strain of Mus musculus molossinus

KW - Reverse transcriptase-polymerase chain reaction (RT-PCR)

KW - Spleen

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JO - In Vivo

JF - In Vivo

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IS - 2

ER -

Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus

Abstract

Keywords

ASJC Scopus subject areas

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