Abstract
The 5′-upsteam region of the genomic gene and cDNA encoding interleukin 4 (IL-4) has been isolated and sequenced from the inbred mouse strain MSKR derived from a Japanese wild mouse using polymerase chain reaction (PCR) methodology. The cDNA sequence of IL-4 of MSKR was found to contain several nucleotide alterations, which result in amino acid substitutions, in comparison with that of inbred mouse BALB/c. In MSKR, IL-4 was expressed at high levels in thymus and spleen as revealed by reverse transcriptase PCR method and Northern hybridization. The tissue-specific expression profile was quite similar to that of laboratory mice.
| Original language | English |
|---|---|
| Pages (from-to) | 277-280 |
| Number of pages | 4 |
| Journal | In Vivo |
| Volume | 23 |
| Issue number | 2 |
| Publication status | Published - 2009 Mar |
| Externally published | Yes |
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Keywords
- 5′-upstream region
- cDNA
- Interleukin 4(IL-4)Thymus
- MSKR inbred strain of Mus musculus molossinus
- Reverse transcriptase-polymerase chain reaction (RT-PCR)
- Spleen
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology
Cite this
Ikeshima-Kataoka, H., Wada, A., Ishiwata, K., Watanabe, N., & Saito, S. (2009). Cloning and expression of cDNA for interleukin 4 from the MSKR inbred strain of Mus musculus molossinus. In Vivo, 23(2), 277-280.