Cloning and nucleotide sequencing of two insecticidal δ- endotoxin genes from bacillus thuringiensis var. Kurstaki hd-1 dna

Shin Ichi Kondo, Nobuya Tamura, Akane Kunitate, Masahira Hattori, Akira Akashi, Iwao Ohmori

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The insecticidal δ-endotoxin genes cry-1-1 and cry-1-2 derived from Bacillus thuringiensis var. kurstaki HD-1 were cloned, and their nucleotide sequences were determined. The nucleotide sequence of cry-1-1 was identical with the one reported by Schnepf et al. except for one base in the coding region. The coding regions of our two cloned toxin genes were not identical, but showed homology. More intensive variations in the sequences were observed in some regions, predominantly in the center of the coding region, 3’-terminal portion and 3-flanking region. The differences in nucleotide sequences may have been mainly induced by base exchanges and deletions. Both translated products in E. coli were toxic to Bombyx mori larvae, but their insecticidal activities were considerably different.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalAgricultural and Biological Chemistry
Volume51
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Bacillus thuringiensis subsp. kurstaki
Bacillus thuringiensis
Cloning
Bacilli
endotoxins
Endotoxins
Organism Cloning
molecular cloning
Nucleotides
Genes
nucleotides
nucleotide sequences
3' Flanking Region
Bombyx
genes
Poisons
Bombyx mori
Escherichia coli
Larva
toxins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cloning and nucleotide sequencing of two insecticidal δ- endotoxin genes from bacillus thuringiensis var. Kurstaki hd-1 dna. / Kondo, Shin Ichi; Tamura, Nobuya; Kunitate, Akane; Hattori, Masahira; Akashi, Akira; Ohmori, Iwao.

In: Agricultural and Biological Chemistry, Vol. 51, No. 2, 1987, p. 455-463.

Research output: Contribution to journalArticle

Kondo, Shin Ichi ; Tamura, Nobuya ; Kunitate, Akane ; Hattori, Masahira ; Akashi, Akira ; Ohmori, Iwao. / Cloning and nucleotide sequencing of two insecticidal δ- endotoxin genes from bacillus thuringiensis var. Kurstaki hd-1 dna. In: Agricultural and Biological Chemistry. 1987 ; Vol. 51, No. 2. pp. 455-463.
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