Abstract
The insecticidal δ-endotoxin genes cry-1-1 and cry-1-2 derived from Bacillus thuringiensis var. kurstaki HD-1 were cloned, and their nucleotide sequences were determined. The nucleotide sequence of cry-1-1 was identical with the one reported by Schnepf et al. except for one base in the coding region. The coding regions of our two cloned toxin genes were not identical, but showed homology. More intensive variations in the sequences were observed in some regions, predominantly in the center of the coding region, 3’-terminal portion and 3’-flanking region. The differences in nucleotide sequences may have been mainly induced by base exchanges and deletions. Both translated products in E. coli were toxic to Bombyx mori larvae, but their insecticidal activities were considerably different.
| Original language | English |
|---|---|
| Pages (from-to) | 455-463 |
| Number of pages | 9 |
| Journal | Agricultural and Biological Chemistry |
| Volume | 51 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Cloning and nucleotide sequencing of two insecticidal δ- endotoxin genes from bacillus thuringiensis var. Kurstaki hd-1 dna. / Kondo, Shin Ichi; Tamura, Nobuya; Kunitate, Akane; Hattori, Masahira; Akashi, Akira; Ohmori, Iwao.
In: Agricultural and Biological Chemistry, Vol. 51, No. 2, 1987, p. 455-463.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and nucleotide sequencing of two insecticidal δ- endotoxin genes from bacillus thuringiensis var. Kurstaki hd-1 dna
AU - Kondo, Shin Ichi
AU - Tamura, Nobuya
AU - Kunitate, Akane
AU - Hattori, Masahira
AU - Akashi, Akira
AU - Ohmori, Iwao
PY - 1987
Y1 - 1987
N2 - The insecticidal δ-endotoxin genes cry-1-1 and cry-1-2 derived from Bacillus thuringiensis var. kurstaki HD-1 were cloned, and their nucleotide sequences were determined. The nucleotide sequence of cry-1-1 was identical with the one reported by Schnepf et al. except for one base in the coding region. The coding regions of our two cloned toxin genes were not identical, but showed homology. More intensive variations in the sequences were observed in some regions, predominantly in the center of the coding region, 3’-terminal portion and 3’-flanking region. The differences in nucleotide sequences may have been mainly induced by base exchanges and deletions. Both translated products in E. coli were toxic to Bombyx mori larvae, but their insecticidal activities were considerably different.
AB - The insecticidal δ-endotoxin genes cry-1-1 and cry-1-2 derived from Bacillus thuringiensis var. kurstaki HD-1 were cloned, and their nucleotide sequences were determined. The nucleotide sequence of cry-1-1 was identical with the one reported by Schnepf et al. except for one base in the coding region. The coding regions of our two cloned toxin genes were not identical, but showed homology. More intensive variations in the sequences were observed in some regions, predominantly in the center of the coding region, 3’-terminal portion and 3’-flanking region. The differences in nucleotide sequences may have been mainly induced by base exchanges and deletions. Both translated products in E. coli were toxic to Bombyx mori larvae, but their insecticidal activities were considerably different.
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UR - http://www.scopus.com/inward/citedby.url?scp=0000838457&partnerID=8YFLogxK
U2 - 10.1080/00021369.1987.10868034
DO - 10.1080/00021369.1987.10868034
M3 - Article
AN - SCOPUS:0000838457
VL - 51
SP - 455
EP - 463
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -