Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12

Tsuyoshi Arai, Satoshi Yusa, Kohtaro Kirimura, Shoji Usami

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalFEMS Microbiology Letters
Volume152
Issue number1
DOIs
Publication statusPublished - 1997 Jul 1

Keywords

  • Lipase
  • Trichosporon fermentans
  • cDNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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