Abstract
A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.
| Original language | English |
|---|---|
| Pages (from-to) | 183-188 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 152 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1997 Jul 1 |
Keywords
- Lipase
- Trichosporon fermentans
- cDNA
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics
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Arai, T., Yusa, S., Kirimura, K., & Usami, S. (1997). Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12. FEMS Microbiology Letters, 152(1), 183-188. https://doi.org/10.1016/S0378-1097(97)00201-2