Abstract
A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.
| Original language | English |
|---|---|
| Pages (from-to) | 183-188 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 152 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1997 Jul 1 |
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Keywords
- cDNA
- Lipase
- Trichosporon fermentans
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12. / Arai, Tsuyoshi; Yusa, Satoshi; Kirimura, Kotaro; Usami, Shoji.
In: FEMS Microbiology Letters, Vol. 152, No. 1, 01.07.1997, p. 183-188.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12
AU - Arai, Tsuyoshi
AU - Yusa, Satoshi
AU - Kirimura, Kotaro
AU - Usami, Shoji
PY - 1997/7/1
Y1 - 1997/7/1
N2 - A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.
AB - A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.
KW - cDNA
KW - Lipase
KW - Trichosporon fermentans
UR - http://www.scopus.com/inward/record.url?scp=0030788939&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030788939&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(97)00201-2
DO - 10.1016/S0378-1097(97)00201-2
M3 - Article
C2 - 9228786
AN - SCOPUS:0030788939
VL - 152
SP - 183
EP - 188
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -