Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12

Tsuyoshi Arai, Satoshi Yusa, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.

    Original languageEnglish
    Pages (from-to)183-188
    Number of pages6
    JournalFEMS Microbiology Letters
    Volume152
    Issue number1
    DOIs
    Publication statusPublished - 1997 Jul 1

    Fingerprint

    Trichosporon
    Lipase
    Organism Cloning
    Complementary DNA
    Amino Acid Sequence
    Geotrichum
    Amino Acids
    Protein Sorting Signals
    Gene Library
    Oligonucleotides
    Clone Cells
    Polymerase Chain Reaction
    Enzymes
    Genes
    Proteins

    Keywords

    • cDNA
    • Lipase
    • Trichosporon fermentans

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Microbiology

    Cite this

    Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12. / Arai, Tsuyoshi; Yusa, Satoshi; Kirimura, Kotaro; Usami, Shoji.

    In: FEMS Microbiology Letters, Vol. 152, No. 1, 01.07.1997, p. 183-188.

    Research output: Contribution to journalArticle

    Arai, Tsuyoshi ; Yusa, Satoshi ; Kirimura, Kotaro ; Usami, Shoji. / Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12. In: FEMS Microbiology Letters. 1997 ; Vol. 152, No. 1. pp. 183-188.
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