Cloning of a cDNA encoding a DNA-binding protein TAXREB302 that is specific for the tax-responsive enhancer of HTLV-I

Hiroshi Nyunoya, Morita Toshiro, Sato Takako, Honma Saeko, Tsujimoto Atsumi, Shimotohno Kunitada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The transcriptional activator, Tax, of human T-cell leukemia virus (HTLV-I) has been considered to interact with cellular proteins to act on target enhancer motifs. Using oligodeoxyribonucleotides containing the tax-responsive element (TAXRE) of the HTLV-I enhancer, we have cloned multiple cDNAs coding for TAXRE-binding proteins (TAXREB), and determined the cDNA and the deduced 200-amino-acid sequences for TAXREB302. The recombinant protein binds to the enhancer DNA by specific interaction to the CRE-like sequence. A single 1.8-kb species of mRNA was detected in cultured cells, as well as in normal human tissues, especially brain and skeletal muscle. The 22-kDa native protein was detected in the cultured-cell lysate by immunoblotting analysis. TAXREB302 does not have structural features common to the CRE-binding protein or activating transcription factor (CREB/ATF) family, but has homology to chicken erythroid transcription factor (Eryf 1 or GATA-1), suggesting a possible protein-protein interaction.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalGene
Volume126
Issue number2
DOIs
Publication statusPublished - 1993 Apr 30
Externally publishedYes

Fingerprint

Deltaretrovirus
DNA-Binding Proteins
Organism Cloning
Complementary DNA
Cultured Cells
GATA1 Transcription Factor
Carrier Proteins
Proteins
Activating Transcription Factors
Oligodeoxyribonucleotides
Recombinant Proteins
Immunoblotting
Amino Acid Sequence
Chickens
Skeletal Muscle
Messenger RNA
tax-responsive element-binding protein 302
DNA
Brain

Keywords

  • adult T-cell leukemia
  • cyclic AMP-responsive element
  • enhancer
  • erythroid transcription factor
  • human T-cell leukemia virus type-I
  • Recombinant DNA
  • South-Western cloning
  • trans-activation

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning of a cDNA encoding a DNA-binding protein TAXREB302 that is specific for the tax-responsive enhancer of HTLV-I. / Nyunoya, Hiroshi; Toshiro, Morita; Takako, Sato; Saeko, Honma; Atsumi, Tsujimoto; Kunitada, Shimotohno.

In: Gene, Vol. 126, No. 2, 30.04.1993, p. 251-255.

Research output: Contribution to journalArticle

Nyunoya, Hiroshi ; Toshiro, Morita ; Takako, Sato ; Saeko, Honma ; Atsumi, Tsujimoto ; Kunitada, Shimotohno. / Cloning of a cDNA encoding a DNA-binding protein TAXREB302 that is specific for the tax-responsive enhancer of HTLV-I. In: Gene. 1993 ; Vol. 126, No. 2. pp. 251-255.
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