Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein

Yasuhiko Matsushita, Orie Miyakawa, Masakazu Deguchi, Masamichi Nishiguchi, Hiroshi Nyunoya

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Viral movement through plasmodesmata in host plants depends on the interaction between virus-encoded movement protein (MP) and host proteins. To search for MP-interacting protein (MIP), far-western screening of a tobacco cDNA library was carried out using a recombinant MP of tomato mosaic virus (ToMV) as a probe. One of the positive cDNA clones, designated MIP204, was highly homologous to a class of transcriptional coactivators commonly referred to as multiprotein bridging factor 1 (MBF1). ToMV MP could also bind to the Arabidopsis homologues of MBF1. The recombinant MIP204 bound to MPs of ToMV and a crucifer tobamovirus CTMV-W, but not of cucumber mosaic virus. MPs of ToMV and the related virus may interact with MBF1-like proteins to modulate host gene expression.

Original languageEnglish
Pages (from-to)1531-1532
Number of pages2
JournalJournal of Experimental Botany
Volume53
Issue number373
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Tomato mosaic virus
Mosaic Viruses
Lycopersicon esculentum
Tobacco
Organism Cloning
molecular cloning
tobacco
Complementary DNA
Proteins
proteins
Tobamovirus
Plasmodesmata
Cucumovirus
Viruses
viruses
plasmodesmata
Cucumber mosaic virus
Gene Library
Recombinant Proteins
Arabidopsis

Keywords

  • Movement protein
  • Multiprotein bridging factor 1
  • Tomato mosaic virus
  • Transcriptional coactivator

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein. / Matsushita, Yasuhiko; Miyakawa, Orie; Deguchi, Masakazu; Nishiguchi, Masamichi; Nyunoya, Hiroshi.

In: Journal of Experimental Botany, Vol. 53, No. 373, 2002, p. 1531-1532.

Research output: Contribution to journalArticle

Matsushita, Yasuhiko ; Miyakawa, Orie ; Deguchi, Masakazu ; Nishiguchi, Masamichi ; Nyunoya, Hiroshi. / Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein. In: Journal of Experimental Botany. 2002 ; Vol. 53, No. 373. pp. 1531-1532.
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