Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs

Hideo Iwasaki, M. Takahashi, T. Niimi, O. Yamashita, T. Yaginuma

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In diapausing eggs of the silkworm, Bombyx mori, embryonic cells are arrested at G2 phase. The ability to undertake cell division is resumed in the course of dispause termination caused by such a treatment as acclimation to 5°C. As an initial trial to investigate the relationship between diapause end embryonic cell cycling, we have cloned and sequenced two Bombyx cDNAs encoding two distinct cdc2-related Ser/Thr protein kinases. One (Bm cdc2) encoded a 37.0 kDa protein which had all of the domains characteristic of other Cdc2 kinase. The other (Bcdrk) encoded a 45.1 kDa protein that was most similar to Drosophila and human cdc2-related protein kinases (Dcdrkprotein and PISSRLE kinase). Northern blot analysis was carried out to examine levels of Bm cdc2 end Bcdrk mRNA during embryogenesis of non-diapause eggs. The result demonstrated that the mRNA level of Bm cdc2 appeared to correspond to the activity of nuclear/cellular division in non-diapause eggs, and that the developmental profile in the level of Bcdrk mRNA was somewhat different from that of Bm cdc2 mRNA.

Original languageEnglish
Pages (from-to)131-141
Number of pages11
JournalInsect Molecular Biology
Volume6
Issue number2
Publication statusPublished - 1997 May
Externally publishedYes

Fingerprint

Bombyx
Cloning
protein kinases
Eggs
Organism Cloning
molecular cloning
phosphotransferases (kinases)
Phosphotransferases
Complementary DNA
Messenger RNA
cyclin-dependent kinase
Bombyx mori
silkworms
diapause
Northern blotting
interphase
cell division
acclimation
Drosophila
embryogenesis

Keywords

  • Bombyx mori
  • Cdc2-related kinase
  • cell division
  • embryonic dispause
  • mRNA

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Iwasaki, H., Takahashi, M., Niimi, T., Yamashita, O., & Yaginuma, T. (1997). Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs. Insect Molecular Biology, 6(2), 131-141.

Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs. / Iwasaki, Hideo; Takahashi, M.; Niimi, T.; Yamashita, O.; Yaginuma, T.

In: Insect Molecular Biology, Vol. 6, No. 2, 05.1997, p. 131-141.

Research output: Contribution to journalArticle

Iwasaki, H, Takahashi, M, Niimi, T, Yamashita, O & Yaginuma, T 1997, 'Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs', Insect Molecular Biology, vol. 6, no. 2, pp. 131-141.
Iwasaki, Hideo ; Takahashi, M. ; Niimi, T. ; Yamashita, O. ; Yaginuma, T. / Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs. In: Insect Molecular Biology. 1997 ; Vol. 6, No. 2. pp. 131-141.
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