Cloning of mouse BMAL2 and its daily expression profile in the suprachiasmatic nucleus

A remarkable acceleration of Bmal2 sequence divergence after Bmal gene duplication

Toshiyuki Okano, Momoko Sasaki, Yoshitaka Fukada

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Brain-Muscle-Arnt-Like-protein 2 (BMAL2; Arnt4) (aryl hydrocarbon receptor nuclear translocator) is a recently identified basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor, which contributes to a positive regulation of autoregulatory feedback loop in vertebrate circadian clock systems. In this study, we cloned cDNAs encoding mouse and rat BMAL2 (mBMAL2 and rBMAL2) from mouse midbrain and rat-1 fibroblast cells, respectively. A phylogenetic analysis strongly suggested that vertebrate Bmal1 and Bmal2 genes were generated by a single gene duplication of an ancestral Bmal gene, a vertebrate ortholog of dCyc gene, and that 'BMAL2's putatively termed so far are orthologous. Interestingly, BMAL2 proteins have diverged about 20-fold more rapidly than BMAL1 proteins after the duplication, suggesting an as-yet-unidentified function conserved in BMAL1 but not in BMAL2. mBmal2 mRNA was constitutively expressed throughout the day under light-dark cycle in the mouse hypothalamus containing suprachiasmatic nucleus, the site of the central circadian oscillator in mammals.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalNeuroscience Letters
Volume300
Issue number2
DOIs
Publication statusPublished - 2001 Mar 9
Externally publishedYes

Fingerprint

ARNTL Transcription Factors
Suprachiasmatic Nucleus
Gene Duplication
Vertebrates
Organism Cloning
Aryl Hydrocarbon Receptor Nuclear Translocator
Genes
Circadian Clocks
Photoperiod
Mesencephalon
Hypothalamus
Mammals
Transcription Factors
Complementary DNA
Fibroblasts
Muscles
Messenger RNA
Brain

Keywords

  • bHLH-PAS
  • BMAL
  • Circadian rhythm
  • Gene duplication
  • Mouse
  • Rat
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Cloning of mouse BMAL2 and its daily expression profile in the suprachiasmatic nucleus: A remarkable acceleration of Bmal2 sequence divergence after Bmal gene duplication",
abstract = "Brain-Muscle-Arnt-Like-protein 2 (BMAL2; Arnt4) (aryl hydrocarbon receptor nuclear translocator) is a recently identified basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor, which contributes to a positive regulation of autoregulatory feedback loop in vertebrate circadian clock systems. In this study, we cloned cDNAs encoding mouse and rat BMAL2 (mBMAL2 and rBMAL2) from mouse midbrain and rat-1 fibroblast cells, respectively. A phylogenetic analysis strongly suggested that vertebrate Bmal1 and Bmal2 genes were generated by a single gene duplication of an ancestral Bmal gene, a vertebrate ortholog of dCyc gene, and that 'BMAL2's putatively termed so far are orthologous. Interestingly, BMAL2 proteins have diverged about 20-fold more rapidly than BMAL1 proteins after the duplication, suggesting an as-yet-unidentified function conserved in BMAL1 but not in BMAL2. mBmal2 mRNA was constitutively expressed throughout the day under light-dark cycle in the mouse hypothalamus containing suprachiasmatic nucleus, the site of the central circadian oscillator in mammals.",
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AU - Fukada, Yoshitaka

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