Abstract
Brain-Muscle-Arnt-Like-protein 2 (BMAL2; Arnt4) (aryl hydrocarbon receptor nuclear translocator) is a recently identified basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor, which contributes to a positive regulation of autoregulatory feedback loop in vertebrate circadian clock systems. In this study, we cloned cDNAs encoding mouse and rat BMAL2 (mBMAL2 and rBMAL2) from mouse midbrain and rat-1 fibroblast cells, respectively. A phylogenetic analysis strongly suggested that vertebrate Bmal1 and Bmal2 genes were generated by a single gene duplication of an ancestral Bmal gene, a vertebrate ortholog of dCyc gene, and that 'BMAL2's putatively termed so far are orthologous. Interestingly, BMAL2 proteins have diverged about 20-fold more rapidly than BMAL1 proteins after the duplication, suggesting an as-yet-unidentified function conserved in BMAL1 but not in BMAL2. mBmal2 mRNA was constitutively expressed throughout the day under light-dark cycle in the mouse hypothalamus containing suprachiasmatic nucleus, the site of the central circadian oscillator in mammals.
| Original language | English |
|---|---|
| Pages (from-to) | 111-114 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 300 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2001 Mar 9 |
| Externally published | Yes |
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Keywords
- bHLH-PAS
- BMAL
- Circadian rhythm
- Gene duplication
- Mouse
- Rat
- Suprachiasmatic nucleus
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Cloning of mouse BMAL2 and its daily expression profile in the suprachiasmatic nucleus : A remarkable acceleration of Bmal2 sequence divergence after Bmal gene duplication. / Okano, Toshiyuki; Sasaki, Momoko; Fukada, Yoshitaka.
In: Neuroscience Letters, Vol. 300, No. 2, 09.03.2001, p. 111-114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning of mouse BMAL2 and its daily expression profile in the suprachiasmatic nucleus
T2 - A remarkable acceleration of Bmal2 sequence divergence after Bmal gene duplication
AU - Okano, Toshiyuki
AU - Sasaki, Momoko
AU - Fukada, Yoshitaka
PY - 2001/3/9
Y1 - 2001/3/9
N2 - Brain-Muscle-Arnt-Like-protein 2 (BMAL2; Arnt4) (aryl hydrocarbon receptor nuclear translocator) is a recently identified basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor, which contributes to a positive regulation of autoregulatory feedback loop in vertebrate circadian clock systems. In this study, we cloned cDNAs encoding mouse and rat BMAL2 (mBMAL2 and rBMAL2) from mouse midbrain and rat-1 fibroblast cells, respectively. A phylogenetic analysis strongly suggested that vertebrate Bmal1 and Bmal2 genes were generated by a single gene duplication of an ancestral Bmal gene, a vertebrate ortholog of dCyc gene, and that 'BMAL2's putatively termed so far are orthologous. Interestingly, BMAL2 proteins have diverged about 20-fold more rapidly than BMAL1 proteins after the duplication, suggesting an as-yet-unidentified function conserved in BMAL1 but not in BMAL2. mBmal2 mRNA was constitutively expressed throughout the day under light-dark cycle in the mouse hypothalamus containing suprachiasmatic nucleus, the site of the central circadian oscillator in mammals.
AB - Brain-Muscle-Arnt-Like-protein 2 (BMAL2; Arnt4) (aryl hydrocarbon receptor nuclear translocator) is a recently identified basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor, which contributes to a positive regulation of autoregulatory feedback loop in vertebrate circadian clock systems. In this study, we cloned cDNAs encoding mouse and rat BMAL2 (mBMAL2 and rBMAL2) from mouse midbrain and rat-1 fibroblast cells, respectively. A phylogenetic analysis strongly suggested that vertebrate Bmal1 and Bmal2 genes were generated by a single gene duplication of an ancestral Bmal gene, a vertebrate ortholog of dCyc gene, and that 'BMAL2's putatively termed so far are orthologous. Interestingly, BMAL2 proteins have diverged about 20-fold more rapidly than BMAL1 proteins after the duplication, suggesting an as-yet-unidentified function conserved in BMAL1 but not in BMAL2. mBmal2 mRNA was constitutively expressed throughout the day under light-dark cycle in the mouse hypothalamus containing suprachiasmatic nucleus, the site of the central circadian oscillator in mammals.
KW - bHLH-PAS
KW - BMAL
KW - Circadian rhythm
KW - Gene duplication
KW - Mouse
KW - Rat
KW - Suprachiasmatic nucleus
UR - http://www.scopus.com/inward/record.url?scp=0035831350&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035831350&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(01)01581-6
DO - 10.1016/S0304-3940(01)01581-6
M3 - Article
VL - 300
SP - 111
EP - 114
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -