Cloning, sequencing and expressing the carotenoid biosynthesis genes, lycopene cyclase and phytoene desaturase, from the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 in Escherichia coli

Haruo Matsumura, Haruko Takeyama, Etsuko Kusakabe, J. Grant Burgess, Tadashi Matsunaga

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Two genes which encode the enzymes lycopene cyclase and phytoene desaturase in the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 have been cloned and sequenced. The gene for lycopene cyclase, designated crtY, was expressed in a strain of Escherichia coli which contained the crtE, B, I and Z genes encoding geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, and β-carotene hydroxylase, respectively. As a result, zeaxanthin production was observed in E. coli transformants. In addition, expression of the E. longus gene crtI for phytoene desaturase in E. coli containing crtE and B resulted in the accumulation of lycopene in transformants. Zeaxanthin and lycopene were also determined by mass spectrum. Nucleotide sequence similarities between E. longus crtY gene and other microbial lycopene cyclase genes are 40.2% (Erwinia herbicola), 37.4% (Erwinia uredovora) and 22.9% (Synechococcus sp.), and those between phytoene desaturase genes are 50.3% (E. herbicola), 54.7% (E. uredovora) and 39.6% (Rhodobacter capsulatus).

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalGene
Volume189
Issue number2
DOIs
Publication statusPublished - 1997 Apr 21
Externally publishedYes

Fingerprint

Sphingomonadaceae
Aerobic Bacteria
Carotenoids
Organism Cloning
Escherichia coli
Erwinia
Genes
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
Microbial Genes
Rhodobacter capsulatus
Synechococcus
Mixed Function Oxygenases
lycopene cyclase-isomerase
phytoene dehydrogenase
Enzymes

Keywords

  • crtI
  • crtY
  • Lycopene
  • β-carotene

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning, sequencing and expressing the carotenoid biosynthesis genes, lycopene cyclase and phytoene desaturase, from the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 in Escherichia coli. / Matsumura, Haruo; Takeyama, Haruko; Kusakabe, Etsuko; Burgess, J. Grant; Matsunaga, Tadashi.

In: Gene, Vol. 189, No. 2, 21.04.1997, p. 169-174.

Research output: Contribution to journalArticle

@article{89d37231ce35418394d4a6951492f69c,
title = "Cloning, sequencing and expressing the carotenoid biosynthesis genes, lycopene cyclase and phytoene desaturase, from the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 in Escherichia coli",
abstract = "Two genes which encode the enzymes lycopene cyclase and phytoene desaturase in the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 have been cloned and sequenced. The gene for lycopene cyclase, designated crtY, was expressed in a strain of Escherichia coli which contained the crtE, B, I and Z genes encoding geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, and β-carotene hydroxylase, respectively. As a result, zeaxanthin production was observed in E. coli transformants. In addition, expression of the E. longus gene crtI for phytoene desaturase in E. coli containing crtE and B resulted in the accumulation of lycopene in transformants. Zeaxanthin and lycopene were also determined by mass spectrum. Nucleotide sequence similarities between E. longus crtY gene and other microbial lycopene cyclase genes are 40.2{\%} (Erwinia herbicola), 37.4{\%} (Erwinia uredovora) and 22.9{\%} (Synechococcus sp.), and those between phytoene desaturase genes are 50.3{\%} (E. herbicola), 54.7{\%} (E. uredovora) and 39.6{\%} (Rhodobacter capsulatus).",
keywords = "crtI, crtY, Lycopene, β-carotene",
author = "Haruo Matsumura and Haruko Takeyama and Etsuko Kusakabe and Burgess, {J. Grant} and Tadashi Matsunaga",
year = "1997",
month = "4",
day = "21",
doi = "10.1016/S0378-1119(96)00788-3",
language = "English",
volume = "189",
pages = "169--174",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Cloning, sequencing and expressing the carotenoid biosynthesis genes, lycopene cyclase and phytoene desaturase, from the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 in Escherichia coli

AU - Matsumura, Haruo

AU - Takeyama, Haruko

AU - Kusakabe, Etsuko

AU - Burgess, J. Grant

AU - Matsunaga, Tadashi

PY - 1997/4/21

Y1 - 1997/4/21

N2 - Two genes which encode the enzymes lycopene cyclase and phytoene desaturase in the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 have been cloned and sequenced. The gene for lycopene cyclase, designated crtY, was expressed in a strain of Escherichia coli which contained the crtE, B, I and Z genes encoding geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, and β-carotene hydroxylase, respectively. As a result, zeaxanthin production was observed in E. coli transformants. In addition, expression of the E. longus gene crtI for phytoene desaturase in E. coli containing crtE and B resulted in the accumulation of lycopene in transformants. Zeaxanthin and lycopene were also determined by mass spectrum. Nucleotide sequence similarities between E. longus crtY gene and other microbial lycopene cyclase genes are 40.2% (Erwinia herbicola), 37.4% (Erwinia uredovora) and 22.9% (Synechococcus sp.), and those between phytoene desaturase genes are 50.3% (E. herbicola), 54.7% (E. uredovora) and 39.6% (Rhodobacter capsulatus).

AB - Two genes which encode the enzymes lycopene cyclase and phytoene desaturase in the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 have been cloned and sequenced. The gene for lycopene cyclase, designated crtY, was expressed in a strain of Escherichia coli which contained the crtE, B, I and Z genes encoding geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, and β-carotene hydroxylase, respectively. As a result, zeaxanthin production was observed in E. coli transformants. In addition, expression of the E. longus gene crtI for phytoene desaturase in E. coli containing crtE and B resulted in the accumulation of lycopene in transformants. Zeaxanthin and lycopene were also determined by mass spectrum. Nucleotide sequence similarities between E. longus crtY gene and other microbial lycopene cyclase genes are 40.2% (Erwinia herbicola), 37.4% (Erwinia uredovora) and 22.9% (Synechococcus sp.), and those between phytoene desaturase genes are 50.3% (E. herbicola), 54.7% (E. uredovora) and 39.6% (Rhodobacter capsulatus).

KW - crtI

KW - crtY

KW - Lycopene

KW - β-carotene

UR - http://www.scopus.com/inward/record.url?scp=0030902310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030902310&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(96)00788-3

DO - 10.1016/S0378-1119(96)00788-3

M3 - Article

C2 - 9168123

AN - SCOPUS:0030902310

VL - 189

SP - 169

EP - 174

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -