Co-ordinated functions of Mms proteins define the surface structure of cubo-octahedral magnetite crystals in magnetotactic bacteria

Atsushi Arakaki, Ayana Yamagishi, Ayumi Fukuyo, Masayoshi Tanaka, Tadashi Matsunaga

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Magnetotactic bacteria synthesize magnetosomes comprised of membrane-enveloped single crystalline magnetite (Fe3O4). The size and morphology of the nano-sized magnetite crystals (<100nm) are highly regulated and bacterial species dependent. However, the control mechanisms of magnetite crystal morphology remain largely unknown. The group of proteins, called Mms (Mms5, Mms6, Mms7, and Mms13), was previously isolated from the surface of cubo-octahedral magnetite crystals in Magnetospirillum magneticum strain AMB-1. Analysis of an mms6 gene deletion mutant suggested that the Mms6 protein plays a major role in the regulation of magnetite crystal size and morphology. In this study, we constructed various mms gene deletion mutants and characterized the magnetite crystals formed by the mutant strains. Comparative analysis showed that all mms genes were involved in the promotion of crystal growth in different manners. The phenotypic characterization of magnetites also suggested that these proteins are involved in controlling the geometries of the crystal surface structures. Thus, the co-ordinated functions of Mms proteins regulate the morphology of the cubo-octahedral magnetite crystals in magnetotactic bacteria.

Original languageEnglish
Pages (from-to)554-567
Number of pages14
JournalMolecular Microbiology
Volume93
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Ferrosoferric Oxide
Membrane Proteins
Bacteria
Gene Deletion
Magnetospirillum
Magnetosomes
Proteins
Crystallization
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology
  • Medicine(all)

Cite this

Co-ordinated functions of Mms proteins define the surface structure of cubo-octahedral magnetite crystals in magnetotactic bacteria. / Arakaki, Atsushi; Yamagishi, Ayana; Fukuyo, Ayumi; Tanaka, Masayoshi; Matsunaga, Tadashi.

In: Molecular Microbiology, Vol. 93, No. 3, 2014, p. 554-567.

Research output: Contribution to journalArticle

Arakaki, Atsushi ; Yamagishi, Ayana ; Fukuyo, Ayumi ; Tanaka, Masayoshi ; Matsunaga, Tadashi. / Co-ordinated functions of Mms proteins define the surface structure of cubo-octahedral magnetite crystals in magnetotactic bacteria. In: Molecular Microbiology. 2014 ; Vol. 93, No. 3. pp. 554-567.
@article{29d1bb3db51948e78ef040a547ad3cc1,
title = "Co-ordinated functions of Mms proteins define the surface structure of cubo-octahedral magnetite crystals in magnetotactic bacteria",
abstract = "Magnetotactic bacteria synthesize magnetosomes comprised of membrane-enveloped single crystalline magnetite (Fe3O4). The size and morphology of the nano-sized magnetite crystals (<100nm) are highly regulated and bacterial species dependent. However, the control mechanisms of magnetite crystal morphology remain largely unknown. The group of proteins, called Mms (Mms5, Mms6, Mms7, and Mms13), was previously isolated from the surface of cubo-octahedral magnetite crystals in Magnetospirillum magneticum strain AMB-1. Analysis of an mms6 gene deletion mutant suggested that the Mms6 protein plays a major role in the regulation of magnetite crystal size and morphology. In this study, we constructed various mms gene deletion mutants and characterized the magnetite crystals formed by the mutant strains. Comparative analysis showed that all mms genes were involved in the promotion of crystal growth in different manners. The phenotypic characterization of magnetites also suggested that these proteins are involved in controlling the geometries of the crystal surface structures. Thus, the co-ordinated functions of Mms proteins regulate the morphology of the cubo-octahedral magnetite crystals in magnetotactic bacteria.",
author = "Atsushi Arakaki and Ayana Yamagishi and Ayumi Fukuyo and Masayoshi Tanaka and Tadashi Matsunaga",
year = "2014",
doi = "10.1111/mmi.12683",
language = "English",
volume = "93",
pages = "554--567",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Co-ordinated functions of Mms proteins define the surface structure of cubo-octahedral magnetite crystals in magnetotactic bacteria

AU - Arakaki, Atsushi

AU - Yamagishi, Ayana

AU - Fukuyo, Ayumi

AU - Tanaka, Masayoshi

AU - Matsunaga, Tadashi

PY - 2014

Y1 - 2014

N2 - Magnetotactic bacteria synthesize magnetosomes comprised of membrane-enveloped single crystalline magnetite (Fe3O4). The size and morphology of the nano-sized magnetite crystals (<100nm) are highly regulated and bacterial species dependent. However, the control mechanisms of magnetite crystal morphology remain largely unknown. The group of proteins, called Mms (Mms5, Mms6, Mms7, and Mms13), was previously isolated from the surface of cubo-octahedral magnetite crystals in Magnetospirillum magneticum strain AMB-1. Analysis of an mms6 gene deletion mutant suggested that the Mms6 protein plays a major role in the regulation of magnetite crystal size and morphology. In this study, we constructed various mms gene deletion mutants and characterized the magnetite crystals formed by the mutant strains. Comparative analysis showed that all mms genes were involved in the promotion of crystal growth in different manners. The phenotypic characterization of magnetites also suggested that these proteins are involved in controlling the geometries of the crystal surface structures. Thus, the co-ordinated functions of Mms proteins regulate the morphology of the cubo-octahedral magnetite crystals in magnetotactic bacteria.

AB - Magnetotactic bacteria synthesize magnetosomes comprised of membrane-enveloped single crystalline magnetite (Fe3O4). The size and morphology of the nano-sized magnetite crystals (<100nm) are highly regulated and bacterial species dependent. However, the control mechanisms of magnetite crystal morphology remain largely unknown. The group of proteins, called Mms (Mms5, Mms6, Mms7, and Mms13), was previously isolated from the surface of cubo-octahedral magnetite crystals in Magnetospirillum magneticum strain AMB-1. Analysis of an mms6 gene deletion mutant suggested that the Mms6 protein plays a major role in the regulation of magnetite crystal size and morphology. In this study, we constructed various mms gene deletion mutants and characterized the magnetite crystals formed by the mutant strains. Comparative analysis showed that all mms genes were involved in the promotion of crystal growth in different manners. The phenotypic characterization of magnetites also suggested that these proteins are involved in controlling the geometries of the crystal surface structures. Thus, the co-ordinated functions of Mms proteins regulate the morphology of the cubo-octahedral magnetite crystals in magnetotactic bacteria.

UR - http://www.scopus.com/inward/record.url?scp=84904987210&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904987210&partnerID=8YFLogxK

U2 - 10.1111/mmi.12683

DO - 10.1111/mmi.12683

M3 - Article

C2 - 24961165

AN - SCOPUS:84904987210

VL - 93

SP - 554

EP - 567

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 3

ER -