TY - JOUR
T1 - Codon reading patterns in Drosophila melanogaster mitochondria based on their tRNA sequences
T2 - A unique wobble rule in animal mitochondria
AU - Tomita, Kozo
AU - Ueda, Takuya
AU - Ishiwa, Sadao
AU - Crain, Pamela F.
AU - McCloskey, James A.
AU - Watanabe, Kimitsuna
N1 - Funding Information:
We thank Dr Y. Watanabe (presently at Dalhousie University, Canada), Messrs Ohkubo and Matsuyama for showing us unpublished sequence data of starfish mt tRNAMet and tRNAIle, and for valuable discussions. We also thank Dr T. Suzuki for showing us the unpublished mass spectrum of the unknown modified uridine. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports and Culture of Japan (K.W.), by the Human Frontiers of Science Program Organization (K.W. and J.A.M.) and by National Institutes of Health Grant GM29812 (J.A.M.).
PY - 1999/11/1
Y1 - 1999/11/1
N2 - Mitochondrial (mt) tRNA(Trp), tRNA(Ile), tRNA(Met), tRNA(Ser)GCU, tRNA(Asn) and tRNA(Lys) were purified from Drosophila melanogaster (fruit fly) and their nucleotide sequences were determined. tRNA(Lys) corresponding to both AAA and AAG lysine codons was found to contain the anticodon CUU, C34 at the wobble position being unmodified. tRNA(Met) corresponding to both AUA and AUG methionine codons was found to contain 5-formylcytidine (f5C) at the wobble position, although the extent of modification is partial. These results suggest that both C and f5C as the wobble bases at the anticodon first position (position 34) can recognize A at the codon third position (position 3) in the fruit fly mt translation system. tRNA(Ser)GCU corresponding to AGU, AGC and AGA serine codons was found to contain unmodified G at the anticodon wobble position, suggesting the utilization of an unconventional G34-A3 base pair during translation. When these tRNA anticodon sequences are compared with those of other animal counterparts, it is concluded that either unmodified C or G at the wobble position can recognize A at the codon third position and that modification from A to t6A at position 37, 3'-adjacent to the anticodon, seems to be important for tRNA possessing C34 to recognize A3 in the mRNA in the fruit fly mt translation system.
AB - Mitochondrial (mt) tRNA(Trp), tRNA(Ile), tRNA(Met), tRNA(Ser)GCU, tRNA(Asn) and tRNA(Lys) were purified from Drosophila melanogaster (fruit fly) and their nucleotide sequences were determined. tRNA(Lys) corresponding to both AAA and AAG lysine codons was found to contain the anticodon CUU, C34 at the wobble position being unmodified. tRNA(Met) corresponding to both AUA and AUG methionine codons was found to contain 5-formylcytidine (f5C) at the wobble position, although the extent of modification is partial. These results suggest that both C and f5C as the wobble bases at the anticodon first position (position 34) can recognize A at the codon third position (position 3) in the fruit fly mt translation system. tRNA(Ser)GCU corresponding to AGU, AGC and AGA serine codons was found to contain unmodified G at the anticodon wobble position, suggesting the utilization of an unconventional G34-A3 base pair during translation. When these tRNA anticodon sequences are compared with those of other animal counterparts, it is concluded that either unmodified C or G at the wobble position can recognize A at the codon third position and that modification from A to t6A at position 37, 3'-adjacent to the anticodon, seems to be important for tRNA possessing C34 to recognize A3 in the mRNA in the fruit fly mt translation system.
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U2 - 10.1093/nar/27.21.4291
DO - 10.1093/nar/27.21.4291
M3 - Article
C2 - 10518623
AN - SCOPUS:0033230183
SN - 0305-1048
VL - 27
SP - 4291
EP - 4297
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 21
ER -