Cofilin-induced unidirectional cooperative conformational changes in actin filaments revealed by high-speed atomic force microscopy

Kien X.uan Ngo, Noriyuki Kodera, Eisaku Katayama, Toshio Ando, Taro Q.P. Uyeda

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

High-speed atomic force microscopy was employed to observe structural changes in actin filaments induced by cofilin binding. Consistent with previous electron and fluorescence microscopic studies, cofilin formed clusters along actin filaments, where the filaments were 2-nm thicker and the helical pitch was ~25% shorter, compared to control filaments. Interestingly, the shortened helical pitch was propagated to the neighboring bare zone on the pointed-end side of the cluster, while the pitch on the barbed-end side was similar to the control. Thus, cofilin clusters induce distinctively asymmetric conformational changes in filaments. Consistent with the idea that cofilin favors actin structures with a shorter helical pitch, cofilin clusters grew unidirectionally toward the pointed-end of the filament. Severing was often observed near the boundaries between bare zones and clusters, but not necessarily at the boundaries.

Original languageEnglish
JournaleLife
Volume4
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • actin
  • biochemistry
  • biophysics
  • cofilin
  • cooperative conformational change
  • dictyostelium
  • human
  • myosin
  • S. cerevisiae
  • structural biology

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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