Cofilin Phosphorylation and Actin Reorganization Activities of Testicular Protein Kinase 2 and Its Predominant Expression in Testicular Sertoli Cells

Jiro Toshima, Junko Y. Toshima, Kazuhide Takeuchi, Reiko Mori, Kensaku Mizuno

    Research output: Contribution to journalArticle

    101 Citations (Scopus)

    Abstract

    We previously identified testicular protein kinase 1 (TESK1), which phosphorylates cofilin and induces actin cytoskeletal reorganization. We now report identification and characterization of another member of a TESK family, testicular protein kinase 2 (TESK2), with 48% amino acid identity with TESK1. Like TESK1, TESK2 phosphorylated cofilin specifically at Ser-3 and induced formation of actin stress fibers and focal adhesions. Both TESK1 and TESK2 are highly expressed in the testis, but in contrast to TESK1, which is predominantly expressed in testicular germ cells, TESK2 is expressed predominantly in nongerminal Sertoli cells. Thus, TESK1 and TESK2 seem to play distinct roles in spermatogenesis. In HeLa cells, TESK1 was localized mainly in the cytoplasm, whereas TESK2 was localized mainly in the nucleus, which means that TESK1 and TESK2 likely have distinct cellular functions. Because the kinase-inactive mutant of TESK2 was localized in the cytoplasm, nuclear/cytoplasmic localization of TESK2 depends on its kinase activity. A TESK2 mutant lacking the C-terminal noncatalytic region had about a 10-fold higher kinase activity in vitro and, when expressed in HeLa cells, induced punctate actin aggregates in the cytoplasm and unusual condensation and fragmentation of nuclei, followed by apoptosis. Thus, we propose that the C-terminal region plays important roles in regulating the kinase activity and cellular functions of TESK2.

    Original languageEnglish
    Pages (from-to)31449-31458
    Number of pages10
    JournalJournal of Biological Chemistry
    Volume276
    Issue number33
    DOIs
    Publication statusPublished - 2001 Aug 17

    Fingerprint

    Actin Depolymerizing Factors
    Phosphorylation
    Sertoli Cells
    Protein Kinases
    Actins
    Phosphotransferases
    Cytoplasm
    HeLa Cells
    Cofilin 2
    Stress Fibers
    Focal Adhesions
    testis-specific protein kinase 1
    Mutant Proteins
    Spermatogenesis
    Germ Cells
    Testis
    Condensation
    Adhesion

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Cofilin Phosphorylation and Actin Reorganization Activities of Testicular Protein Kinase 2 and Its Predominant Expression in Testicular Sertoli Cells. / Toshima, Jiro; Toshima, Junko Y.; Takeuchi, Kazuhide; Mori, Reiko; Mizuno, Kensaku.

    In: Journal of Biological Chemistry, Vol. 276, No. 33, 17.08.2001, p. 31449-31458.

    Research output: Contribution to journalArticle

    Toshima, Jiro ; Toshima, Junko Y. ; Takeuchi, Kazuhide ; Mori, Reiko ; Mizuno, Kensaku. / Cofilin Phosphorylation and Actin Reorganization Activities of Testicular Protein Kinase 2 and Its Predominant Expression in Testicular Sertoli Cells. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 33. pp. 31449-31458.
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