Colocalization of pinopsin with two types of G-protein α-subunits in the chicken pineal gland

A. Matsushita, T. Yoshikawa, Toshiyuki Okano, T. Kasahara, Y. Fukada

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pinopsin is a photoreceptive molecule present in the outer segments of chicken pinealocytes. In this paper, the localization of α-subunits of G-proteins, rod transducin (Gt1) and Gq/11, was examined by immunoelectron microscopy to investigate whether these G-proteins colocalize with pinopsin in the outer segments. Ultrathin sections of the chicken pineal gland were double-immunolabeled with antibodies to pinopsin and either Gt1α or Gq/11α. As shown previously, the outer segments around the follicular lumen exhibited divergent morphology with ciliary, bulbous, or lamellate shapes, and most of them displayed pinopsin immunoreactivity. The majority (> 90%) of pinopsin-immunopositive outer segments were labeled by anti-Gt1α and/or anti-Gq/11α antibodies. Application of double-immunolabeling to serial sections demonstrated that a large number of the pinopsin-immunopositive outer segments contained both Gt1α and Gq/11α immunoreactivities. These results suggest that Gt1α and Gq/11α are functionally coupled with light-activated pinopsin within a single outer segment.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalCell and Tissue Research
Volume299
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Pineal Gland
Protein Subunits
GTP-Binding Proteins
Chickens
Transducin
Immunoelectron Microscopy
Antibodies
Microscopic examination
Light
Molecules

Keywords

  • Chicken
  • Circadian clock
  • Gq/11
  • Immunoelectron microscopy
  • Phototransduction
  • Pinealocyte
  • Rod transducin

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Colocalization of pinopsin with two types of G-protein α-subunits in the chicken pineal gland. / Matsushita, A.; Yoshikawa, T.; Okano, Toshiyuki; Kasahara, T.; Fukada, Y.

In: Cell and Tissue Research, Vol. 299, No. 2, 2000, p. 245-251.

Research output: Contribution to journalArticle

Matsushita, A. ; Yoshikawa, T. ; Okano, Toshiyuki ; Kasahara, T. ; Fukada, Y. / Colocalization of pinopsin with two types of G-protein α-subunits in the chicken pineal gland. In: Cell and Tissue Research. 2000 ; Vol. 299, No. 2. pp. 245-251.
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