Colorimetric microchip assay using our own whole blood collected by a painless needle for home medical care

Madoka Takai, Masao Nagai, Yuji Morimoto, Kunihiko Sasao, Akio Oki, Jun Nakanishi, Hiromichi Inokuchi, Chia Hsien Chang, Jun Kikuchi, Hiroki Ogawa, Yasuhiro Horiike

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have developed a colorimetric measurement chip that measures triglycerides, total cholesterol, and high-density lipoprotein in 6 μL of whole blood collected with a painless needle. The chip can be used by patients to self-monitor certain health conditions at home. This chip contains a sharp 150 μm diameter stainless steel (SUS) needle that collects blood painlessly. The chip consists of three layers of injection-molded poly(methyl methacrylate) bonded together with two double-sided tapes. Two commercial reagents are used, and the volume ratio of plasma to reagent is doubled from the reagent specification to reduce the optical absorption length (and chip mass) by half. Centrifugal force separates the plasma from the blood, and then weighs out and mixes the plasma and reagents. A zigzag channel allows mixing of the plasma with the reagents mainly by vortex motion due to the centrifugal force generated at the corners of the channel. The measured values correlated well with conventionally tested values.

Original languageEnglish
Pages (from-to)6469-6476
Number of pages8
JournalAnalyst
Volume138
Issue number21
DOIs
Publication statusPublished - 2013 Nov 7
Externally publishedYes

Fingerprint

Home Care Services
Health care
Needles
Assays
Blood
blood
assay
Plasmas
plasma
Lipoproteins
Cholesterol
Polymethyl methacrylates
Tapes
Light absorption
Plasma Volume
Stainless Steel
vortex
Polymethyl Methacrylate
Vortex flow
Stainless steel

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Takai, M., Nagai, M., Morimoto, Y., Sasao, K., Oki, A., Nakanishi, J., ... Horiike, Y. (2013). Colorimetric microchip assay using our own whole blood collected by a painless needle for home medical care. Analyst, 138(21), 6469-6476. https://doi.org/10.1039/c3an00881a

Colorimetric microchip assay using our own whole blood collected by a painless needle for home medical care. / Takai, Madoka; Nagai, Masao; Morimoto, Yuji; Sasao, Kunihiko; Oki, Akio; Nakanishi, Jun; Inokuchi, Hiromichi; Chang, Chia Hsien; Kikuchi, Jun; Ogawa, Hiroki; Horiike, Yasuhiro.

In: Analyst, Vol. 138, No. 21, 07.11.2013, p. 6469-6476.

Research output: Contribution to journalArticle

Takai, M, Nagai, M, Morimoto, Y, Sasao, K, Oki, A, Nakanishi, J, Inokuchi, H, Chang, CH, Kikuchi, J, Ogawa, H & Horiike, Y 2013, 'Colorimetric microchip assay using our own whole blood collected by a painless needle for home medical care', Analyst, vol. 138, no. 21, pp. 6469-6476. https://doi.org/10.1039/c3an00881a
Takai M, Nagai M, Morimoto Y, Sasao K, Oki A, Nakanishi J et al. Colorimetric microchip assay using our own whole blood collected by a painless needle for home medical care. Analyst. 2013 Nov 7;138(21):6469-6476. https://doi.org/10.1039/c3an00881a
Takai, Madoka ; Nagai, Masao ; Morimoto, Yuji ; Sasao, Kunihiko ; Oki, Akio ; Nakanishi, Jun ; Inokuchi, Hiromichi ; Chang, Chia Hsien ; Kikuchi, Jun ; Ogawa, Hiroki ; Horiike, Yasuhiro. / Colorimetric microchip assay using our own whole blood collected by a painless needle for home medical care. In: Analyst. 2013 ; Vol. 138, No. 21. pp. 6469-6476.
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