Colorimetric microchip assay using our own whole blood collected by a painless needle for home medical care

Madoka Takai*, Masao Nagai, Yuji Morimoto, Kunihiko Sasao, Akio Oki, Jun Nakanishi, Hiromichi Inokuchi, Chia Hsien Chang, Jun Kikuchi, Hiroki Ogawa, Yasuhiro Horiike

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


We have developed a colorimetric measurement chip that measures triglycerides, total cholesterol, and high-density lipoprotein in 6 μL of whole blood collected with a painless needle. The chip can be used by patients to self-monitor certain health conditions at home. This chip contains a sharp 150 μm diameter stainless steel (SUS) needle that collects blood painlessly. The chip consists of three layers of injection-molded poly(methyl methacrylate) bonded together with two double-sided tapes. Two commercial reagents are used, and the volume ratio of plasma to reagent is doubled from the reagent specification to reduce the optical absorption length (and chip mass) by half. Centrifugal force separates the plasma from the blood, and then weighs out and mixes the plasma and reagents. A zigzag channel allows mixing of the plasma with the reagents mainly by vortex motion due to the centrifugal force generated at the corners of the channel. The measured values correlated well with conventionally tested values.

Original languageEnglish
Pages (from-to)6469-6476
Number of pages8
Issue number21
Publication statusPublished - 2013 Nov 7
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry


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