Combination of polymorphisms in the β2-adrenergic receptor and nitric oxide synthase 3 genes increases the risk for hypertension

Maiko Misono, Seiji Maeda, Motoyuki Iemitsu, Yoshio Nakata, Takeshi Otsuki, Jun Sugawara, Hirofumi Zempo, Mutsuko Yoshizawa, Asako Miyaki, Shinya Kuno, Mitsuo Matsuda, Ryuichi Ajisaka

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Objective: Hypertension is a major risk factor for cardiovascular disease. Polymorphism in the β2-adrenergic receptor (ADRB2) and nitric oxide synthase 3 (NOS3) genes is associated with clinical cardiovascular phenotypes. The Arg16Gly and Glu298Asp polymorphisms of ADRB2 and NOS3 genes, respectively, have been reported to be associated with hypertension. We hypothesized that a combination of these two polymorphisms increases the risk for hypertension. Hence, we examined the effect of this combination of single-nucleotide polymorphisms on the risk for hypertension. Methods: Our cross-sectional study comprised 402 middle-aged and elderly human participants. We determined the genotypes of Arg16Gly and Glu298Asp single-nucleotide polymorphisms in ADRB2 and NOS3, respectively, by TaqMan PCR method; we also measured the resting blood pressure. Results: The odds ratio for the presence of hypertension in individuals having the Gly/Gly genotype of ADRB2 compared with those having the other genotypes (Arg/Arg and Arg/Gly) was 2.87. With regard to the Glu298Asp polymorphism in NOS3, the odds ratio for the presence of hypertension in individuals having the Glu/Glu genotype of NOS3 when compared with those having the other genotypes (Asp/Asp and Asp/Glu) was 2.79. Interestingly, the odds ratio was 7.64 for individuals having a combination of the Gly/Gly genotype of ADRB2 and Glu/Glu genotype of NOS3 when compared with those having a combination of Arg/Arg and Arg/Gly genotypes of ADRB2 and Asp/Asp and Asp/Glu genotypes of NOS3. Conclusion: We revealed that a combination of the Arg16Gly and Glu298Asp polymorphisms in ADRB2 and NOS3, respectively, remarkably increased the risk for hypertension in middle-aged and elderly humans.

Original languageEnglish
Pages (from-to)1377-1383
Number of pages7
JournalJournal of Hypertension
Volume27
Issue number7
DOIs
Publication statusPublished - 2009 Jul
Externally publishedYes

Keywords

  • Combination
  • Hypertension
  • Nitric oxide synthase 3
  • Single-nucleotide polymorphism
  • β-adrenergic receptor

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

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