Common single nucleotide polymorphisms in the FNDC5 gene are associated with glucose metabolism but do not affect serum irisin levels in Japanese men with low fitness levels

Kumpei Tanisawa, Hirokazu Taniguchi, Xiaomin Sun, Tomoko Ito, Zhen Bo Cao, Shizuo Sakamoto, Mitsuru Higuchi

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30 Citations (Scopus)

Abstract

Objective This cross-sectional study analyzed the association of serum irisin concentrations with cardiorespiratory fitness levels and common single nucleotide polymorphisms (SNPs) in the FNDC5 gene and examined the relationships between cardiorespiratory fitness levels, common SNPs in FNDC5, and glucose metabolism. Materials/Methods Cardiorespiratory fitness was assessed by measuring peak oxygen uptake (VO2peak) and serum irisin levels by ELISA in 163 Japanese men (age, 21-79 years). Subjects were divided into low- and high-fitness groups within each age group according to the median VO 2peak value. Common SNPs (rs3480 and rs16835198) of the FNDC5 gene were genotyped with the TaqMan assay. Glucose metabolism was evaluated by measuring HbA1c, fasting plasma glucose (FPG), insulin levels, and HOMA-IR. Results Serum irisin levels were negatively correlated with age (p < 0.001) and not associated with the VO2peak or HOMA-IR. In the low-fitness group, SNP analysis revealed that subjects with the rs3480 AG and GG genotypes had higher levels of insulin and HOMA-IR than those with the AA genotype (p < 0.01; no significant difference was observed in the high-fitness group). The GG genotypes of rs16835198 were associated with increased HbA1c and FPG in the low-fitness group only (p < 0.05). SNPs and both fitness groups were not associated with serum irisin levels. Conclusions In Japanese men, cardiorespiratory fitness levels and common SNPs in FNDC5 are not associated with circulating irisin levels, whereas high cardiorespiratory fitness abolishes the association between the rs3480 and rs16835198 SNPs and glucose metabolism independent of serum irisin levels.

Original languageEnglish
Pages (from-to)574-583
Number of pages10
JournalMetabolism: Clinical and Experimental
Volume63
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Single Nucleotide Polymorphism
Glucose
Serum
Genes
Genotype
Fasting
Insulin
Age Groups
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Cardiorespiratory Fitness
Oxygen

Keywords

  • Cardiorespiratory fitness
  • FNDC5
  • Glucose metabolism
  • Irisin
  • SNP

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{c914bf7452c445a7954fe47d3a0d1f05,
title = "Common single nucleotide polymorphisms in the FNDC5 gene are associated with glucose metabolism but do not affect serum irisin levels in Japanese men with low fitness levels",
abstract = "Objective This cross-sectional study analyzed the association of serum irisin concentrations with cardiorespiratory fitness levels and common single nucleotide polymorphisms (SNPs) in the FNDC5 gene and examined the relationships between cardiorespiratory fitness levels, common SNPs in FNDC5, and glucose metabolism. Materials/Methods Cardiorespiratory fitness was assessed by measuring peak oxygen uptake (VO2peak) and serum irisin levels by ELISA in 163 Japanese men (age, 21-79 years). Subjects were divided into low- and high-fitness groups within each age group according to the median VO 2peak value. Common SNPs (rs3480 and rs16835198) of the FNDC5 gene were genotyped with the TaqMan assay. Glucose metabolism was evaluated by measuring HbA1c, fasting plasma glucose (FPG), insulin levels, and HOMA-IR. Results Serum irisin levels were negatively correlated with age (p < 0.001) and not associated with the VO2peak or HOMA-IR. In the low-fitness group, SNP analysis revealed that subjects with the rs3480 AG and GG genotypes had higher levels of insulin and HOMA-IR than those with the AA genotype (p < 0.01; no significant difference was observed in the high-fitness group). The GG genotypes of rs16835198 were associated with increased HbA1c and FPG in the low-fitness group only (p < 0.05). SNPs and both fitness groups were not associated with serum irisin levels. Conclusions In Japanese men, cardiorespiratory fitness levels and common SNPs in FNDC5 are not associated with circulating irisin levels, whereas high cardiorespiratory fitness abolishes the association between the rs3480 and rs16835198 SNPs and glucose metabolism independent of serum irisin levels.",
keywords = "Cardiorespiratory fitness, FNDC5, Glucose metabolism, Irisin, SNP",
author = "Kumpei Tanisawa and Hirokazu Taniguchi and Xiaomin Sun and Tomoko Ito and Cao, {Zhen Bo} and Shizuo Sakamoto and Mitsuru Higuchi",
year = "2014",
doi = "10.1016/j.metabol.2014.01.005",
language = "English",
volume = "63",
pages = "574--583",
journal = "Metabolism: Clinical and Experimental",
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TY - JOUR

T1 - Common single nucleotide polymorphisms in the FNDC5 gene are associated with glucose metabolism but do not affect serum irisin levels in Japanese men with low fitness levels

AU - Tanisawa, Kumpei

AU - Taniguchi, Hirokazu

AU - Sun, Xiaomin

AU - Ito, Tomoko

AU - Cao, Zhen Bo

AU - Sakamoto, Shizuo

AU - Higuchi, Mitsuru

PY - 2014

Y1 - 2014

N2 - Objective This cross-sectional study analyzed the association of serum irisin concentrations with cardiorespiratory fitness levels and common single nucleotide polymorphisms (SNPs) in the FNDC5 gene and examined the relationships between cardiorespiratory fitness levels, common SNPs in FNDC5, and glucose metabolism. Materials/Methods Cardiorespiratory fitness was assessed by measuring peak oxygen uptake (VO2peak) and serum irisin levels by ELISA in 163 Japanese men (age, 21-79 years). Subjects were divided into low- and high-fitness groups within each age group according to the median VO 2peak value. Common SNPs (rs3480 and rs16835198) of the FNDC5 gene were genotyped with the TaqMan assay. Glucose metabolism was evaluated by measuring HbA1c, fasting plasma glucose (FPG), insulin levels, and HOMA-IR. Results Serum irisin levels were negatively correlated with age (p < 0.001) and not associated with the VO2peak or HOMA-IR. In the low-fitness group, SNP analysis revealed that subjects with the rs3480 AG and GG genotypes had higher levels of insulin and HOMA-IR than those with the AA genotype (p < 0.01; no significant difference was observed in the high-fitness group). The GG genotypes of rs16835198 were associated with increased HbA1c and FPG in the low-fitness group only (p < 0.05). SNPs and both fitness groups were not associated with serum irisin levels. Conclusions In Japanese men, cardiorespiratory fitness levels and common SNPs in FNDC5 are not associated with circulating irisin levels, whereas high cardiorespiratory fitness abolishes the association between the rs3480 and rs16835198 SNPs and glucose metabolism independent of serum irisin levels.

AB - Objective This cross-sectional study analyzed the association of serum irisin concentrations with cardiorespiratory fitness levels and common single nucleotide polymorphisms (SNPs) in the FNDC5 gene and examined the relationships between cardiorespiratory fitness levels, common SNPs in FNDC5, and glucose metabolism. Materials/Methods Cardiorespiratory fitness was assessed by measuring peak oxygen uptake (VO2peak) and serum irisin levels by ELISA in 163 Japanese men (age, 21-79 years). Subjects were divided into low- and high-fitness groups within each age group according to the median VO 2peak value. Common SNPs (rs3480 and rs16835198) of the FNDC5 gene were genotyped with the TaqMan assay. Glucose metabolism was evaluated by measuring HbA1c, fasting plasma glucose (FPG), insulin levels, and HOMA-IR. Results Serum irisin levels were negatively correlated with age (p < 0.001) and not associated with the VO2peak or HOMA-IR. In the low-fitness group, SNP analysis revealed that subjects with the rs3480 AG and GG genotypes had higher levels of insulin and HOMA-IR than those with the AA genotype (p < 0.01; no significant difference was observed in the high-fitness group). The GG genotypes of rs16835198 were associated with increased HbA1c and FPG in the low-fitness group only (p < 0.05). SNPs and both fitness groups were not associated with serum irisin levels. Conclusions In Japanese men, cardiorespiratory fitness levels and common SNPs in FNDC5 are not associated with circulating irisin levels, whereas high cardiorespiratory fitness abolishes the association between the rs3480 and rs16835198 SNPs and glucose metabolism independent of serum irisin levels.

KW - Cardiorespiratory fitness

KW - FNDC5

KW - Glucose metabolism

KW - Irisin

KW - SNP

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U2 - 10.1016/j.metabol.2014.01.005

DO - 10.1016/j.metabol.2014.01.005

M3 - Article

VL - 63

SP - 574

EP - 583

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

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ER -