Comparative analysis of genetic diversity and expression of amoA in wastewater treatment processes

Y. Ebie, N. Noda, H. Miura, M. Matsumura, Satoshi Tsuneda, A. Hirata, Y. Inamori

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the presence of amoA mRNA, were regarded as metabolically active. The DGGE profiles derived from amoA mRNA and from its gene, which were amplified by RT-PCR or PCR using samples collected from a bench-scale reactor treating high concentration of inorganic ammonia, were similar. In contrast, RNA and DNA-derived DGGE profiles from three domestic wastewater treatment facilities were different from each other. These data indicate that the dominant ammonia oxidizers in the bench-scale reactor exhibited ammonia-oxidizing activity, whereas some ammonia oxidizers in the domestic wastewater treatment facilities apparently did not express high levels of amoA mRNA.

    Original languageEnglish
    Pages (from-to)740-744
    Number of pages5
    JournalApplied Microbiology and Biotechnology
    Volume64
    Issue number5
    DOIs
    Publication statusPublished - 2004 Jun

    Fingerprint

    Waste Water
    Ammonia
    Wastewater treatment
    Denaturing Gradient Gel Electrophoresis
    Electrophoresis
    Gels
    Polymerase Chain Reaction
    Messenger RNA
    RNA
    DNA
    Genes
    Population

    ASJC Scopus subject areas

    • Biotechnology
    • Microbiology
    • Bioengineering

    Cite this

    Comparative analysis of genetic diversity and expression of amoA in wastewater treatment processes. / Ebie, Y.; Noda, N.; Miura, H.; Matsumura, M.; Tsuneda, Satoshi; Hirata, A.; Inamori, Y.

    In: Applied Microbiology and Biotechnology, Vol. 64, No. 5, 06.2004, p. 740-744.

    Research output: Contribution to journalArticle

    Ebie, Y. ; Noda, N. ; Miura, H. ; Matsumura, M. ; Tsuneda, Satoshi ; Hirata, A. ; Inamori, Y. / Comparative analysis of genetic diversity and expression of amoA in wastewater treatment processes. In: Applied Microbiology and Biotechnology. 2004 ; Vol. 64, No. 5. pp. 740-744.
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    AU - Hirata, A.

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