Comparative analysis of the expression patterns of UPR-target genes caused by upr-inducing compounds

Satoko Shinjo, Yuji Mizotani, Etsu Tashiro, Masaya Imoto

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Endoplasmic reticulum (ER) stress, due to an accumulation of unfolded proteins in the ER, leads to a process known as the unfolded protein response (UPR). Since the several compounds used to induce UPR have different modes of action, their mechanisms of protein accumulation are thought to be different, but it is unclear whether these compounds can upregulate UPR target genes with similar kinetics. Hence, we sought to compare the expression patterns of nine UPR target genes induced by seven UPR-inducing compounds. Hierarchical clustering analysis revealed that the expression patterns of the UPR target genes induced by the seven compounds were classified into two clusters; cluster A (thapsigargin, tunicamycin, 2-deoxyglucose, and dithiothreitol) and cluster B (brefeldin A, monensin, and eeyarestatin I). Thus, this study suggests the existence of at least two types of UPR target gene expression profiles, which depend on the mode of action of the compounds.

Original languageEnglish
Pages (from-to)729-735
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number4
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Endoplasmic reticulum (ER) stress
  • Profiling
  • Unfolded protein response (UPR)
  • UPR-inducing compound

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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