Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157

H7 Sakai strain and an Escherichia coli K-12 strain MG1655

M. Ohnishi, T. Murata, K. Nakayama, S. Kuhara, Masahira Hattori, K. Kurokawa, T. Yasunaga, K. Yokoyama, K. Makino, H. Shinagawa, T. Hayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Two primer sets for direct sequence determination of all seven rRNA operons (rrn) of Escherichia coli have been developed; one is for specific-amplification of each rrn operon and the other is for direct sequencing of the amplified operons. Using these primer sets, we determined the nucleotide sequences of seven rrn operons, including promoter and terminator regions, of an enterohemorrhagic E. coli (EHEC) O157:H7 Sakai strain. To elucidate the intercistronic or intraspecific variation of rrn operons, their sequences were compared with those for the K-12 rrn operons. The rrn genes and the internal transcribed spacer regions showed a higher Similarity to each other in each strain than between the corresponding operons of the two strains. However, the degree of intercistronic homogeneity was much higher in the EHEC strain than in K-12. In contrast, promoter and terminator regions in each operons were conserved between the corresponding operons of the two strains, which exceeded intercistronic similarity.

Original languageEnglish
Pages (from-to)315-324
Number of pages10
JournalSystematic and Applied Microbiology
Volume23
Issue number3
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

rRNA Operon
Escherichia coli K12
Enterohemorrhagic Escherichia coli
enterohemorrhagic Escherichia coli
Escherichia coli O157
intraspecific variation
operon
Operon
homogeneity
amplification
ribosomal RNA
Escherichia coli
gene
Genetic Terminator Regions
terminator regions
Genetic Promoter Regions
promoter regions
analysis
Sequence Analysis
internal transcribed spacers

Keywords

  • Direct sequencing
  • E. coli K-12
  • Enterohemorrhagic E. coli O157:H7
  • Intercistronic variation
  • Intraspecific variation
  • rrn operon

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157 : H7 Sakai strain and an Escherichia coli K-12 strain MG1655. / Ohnishi, M.; Murata, T.; Nakayama, K.; Kuhara, S.; Hattori, Masahira; Kurokawa, K.; Yasunaga, T.; Yokoyama, K.; Makino, K.; Shinagawa, H.; Hayashi, T.

In: Systematic and Applied Microbiology, Vol. 23, No. 3, 2000, p. 315-324.

Research output: Contribution to journalArticle

Ohnishi, M, Murata, T, Nakayama, K, Kuhara, S, Hattori, M, Kurokawa, K, Yasunaga, T, Yokoyama, K, Makino, K, Shinagawa, H & Hayashi, T 2000, 'Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157: H7 Sakai strain and an Escherichia coli K-12 strain MG1655', Systematic and Applied Microbiology, vol. 23, no. 3, pp. 315-324.
Ohnishi, M. ; Murata, T. ; Nakayama, K. ; Kuhara, S. ; Hattori, Masahira ; Kurokawa, K. ; Yasunaga, T. ; Yokoyama, K. ; Makino, K. ; Shinagawa, H. ; Hayashi, T. / Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157 : H7 Sakai strain and an Escherichia coli K-12 strain MG1655. In: Systematic and Applied Microbiology. 2000 ; Vol. 23, No. 3. pp. 315-324.
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