Comparative chromosome mapping of sex-linked genes and identification of sex chromosomal rearrangements in the Japanese wrinkled frog (Rana rugosa, Ranidae) with ZW and XY sex chromosome systems

Yoshinobu Uno, Chizuko Nishida, Yuki Oshima, Satoshi Yokoyama, Ikuo Miura, Yoichi Matsuda, Masahisa Nakamura

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    There are regional variations of sex chromosome morphologies in the Japanese wrinkled frog, Rana rugosa (2n∈=∈26): heterogametic ZZ/ZW-type and XX/XY-type sex chromosomes, and two different types of homomorphic sex chromosomes. To search for homology between the ZW and XY sex chromosomes and the chromosome rearrangements that have occurred during sex chromosomal differentiation in R. rugosa, we performed chromosome mapping of sexual differentiation genes for R. rugosa by FISH. Three genes, AR, SF-1/Ad4BP and Sox3, were localized to both the ZW and XY chromosomes, and their locations were all different between the Z and W and between the X and Y. AR and SF-1/Ad4BP were located on the short arms of the W and X and the long arms of Z and Y, and Sox3 was mapped to the different locations on the long arms between the Z and W and between the X and Y, probably as a result of multiple rearrangements that occurred during the process of sex chromosome differentiation. However, the chromosomal locations of three genes were almost consistent between the Z and Y and between the W and X, indicating that the Z and Y chromosomes and the W and X chromosomes were respectively derived from the same origins. Dmrt1, which is located on avian sex chromosomes, was localized to autosomes in R. rugosa with both the ZW and XY sex chromosomes, suggesting that Dmrt1 might not be related to sex determination in this species.

    Original languageEnglish
    Pages (from-to)637-647
    Number of pages11
    JournalChromosome Research
    Volume16
    Issue number4
    DOIs
    Publication statusPublished - 2008 Jun

    Fingerprint

    Ranidae
    Sex Chromosomes
    Chromosome Mapping
    Anura
    Sex Differentiation
    Genes
    Chromosomes
    vpr Genes
    Y Chromosome
    X Chromosome

    Keywords

    • Anura
    • Fluorescence in situ hybridization
    • Gene order
    • Homology
    • Linkage
    • Sex chromosome
    • Sex determination

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Comparative chromosome mapping of sex-linked genes and identification of sex chromosomal rearrangements in the Japanese wrinkled frog (Rana rugosa, Ranidae) with ZW and XY sex chromosome systems. / Uno, Yoshinobu; Nishida, Chizuko; Oshima, Yuki; Yokoyama, Satoshi; Miura, Ikuo; Matsuda, Yoichi; Nakamura, Masahisa.

    In: Chromosome Research, Vol. 16, No. 4, 06.2008, p. 637-647.

    Research output: Contribution to journalArticle

    Uno, Yoshinobu ; Nishida, Chizuko ; Oshima, Yuki ; Yokoyama, Satoshi ; Miura, Ikuo ; Matsuda, Yoichi ; Nakamura, Masahisa. / Comparative chromosome mapping of sex-linked genes and identification of sex chromosomal rearrangements in the Japanese wrinkled frog (Rana rugosa, Ranidae) with ZW and XY sex chromosome systems. In: Chromosome Research. 2008 ; Vol. 16, No. 4. pp. 637-647.
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    abstract = "There are regional variations of sex chromosome morphologies in the Japanese wrinkled frog, Rana rugosa (2n∈=∈26): heterogametic ZZ/ZW-type and XX/XY-type sex chromosomes, and two different types of homomorphic sex chromosomes. To search for homology between the ZW and XY sex chromosomes and the chromosome rearrangements that have occurred during sex chromosomal differentiation in R. rugosa, we performed chromosome mapping of sexual differentiation genes for R. rugosa by FISH. Three genes, AR, SF-1/Ad4BP and Sox3, were localized to both the ZW and XY chromosomes, and their locations were all different between the Z and W and between the X and Y. AR and SF-1/Ad4BP were located on the short arms of the W and X and the long arms of Z and Y, and Sox3 was mapped to the different locations on the long arms between the Z and W and between the X and Y, probably as a result of multiple rearrangements that occurred during the process of sex chromosome differentiation. However, the chromosomal locations of three genes were almost consistent between the Z and Y and between the W and X, indicating that the Z and Y chromosomes and the W and X chromosomes were respectively derived from the same origins. Dmrt1, which is located on avian sex chromosomes, was localized to autosomes in R. rugosa with both the ZW and XY sex chromosomes, suggesting that Dmrt1 might not be related to sex determination in this species.",
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    AU - Nishida, Chizuko

    AU - Oshima, Yuki

    AU - Yokoyama, Satoshi

    AU - Miura, Ikuo

    AU - Matsuda, Yoichi

    AU - Nakamura, Masahisa

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    AB - There are regional variations of sex chromosome morphologies in the Japanese wrinkled frog, Rana rugosa (2n∈=∈26): heterogametic ZZ/ZW-type and XX/XY-type sex chromosomes, and two different types of homomorphic sex chromosomes. To search for homology between the ZW and XY sex chromosomes and the chromosome rearrangements that have occurred during sex chromosomal differentiation in R. rugosa, we performed chromosome mapping of sexual differentiation genes for R. rugosa by FISH. Three genes, AR, SF-1/Ad4BP and Sox3, were localized to both the ZW and XY chromosomes, and their locations were all different between the Z and W and between the X and Y. AR and SF-1/Ad4BP were located on the short arms of the W and X and the long arms of Z and Y, and Sox3 was mapped to the different locations on the long arms between the Z and W and between the X and Y, probably as a result of multiple rearrangements that occurred during the process of sex chromosome differentiation. However, the chromosomal locations of three genes were almost consistent between the Z and Y and between the W and X, indicating that the Z and Y chromosomes and the W and X chromosomes were respectively derived from the same origins. Dmrt1, which is located on avian sex chromosomes, was localized to autosomes in R. rugosa with both the ZW and XY sex chromosomes, suggesting that Dmrt1 might not be related to sex determination in this species.

    KW - Anura

    KW - Fluorescence in situ hybridization

    KW - Gene order

    KW - Homology

    KW - Linkage

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    KW - Sex determination

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