Comparative genome analysis of Lactobacillus renteri and Lactobacillus fermentum reveal a genomic Island for reuterin and cobalamin production

Hidetoshi Morita, T. O H Hidehiro, Shinji Fukuda, Hiroshi Horikawa, Kenshiro Oshima, Takehito Suzuki, Masaru Murakami, Shin Hisamatsu, Yukio Kato, Tatsuya Takizawa, Hideo Fukuoka, Tetsuhiko Yoshimura, Kikuji Itoh, Daniel J. O'Sullivan, Larry L. Mckay, Hiroshi Ohno, Jun Kikuchi, Toshio Masaoka, Masahira Hattori

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects of L. reuteri have been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. We determined the complete genome sequences of the reuterin-producing L. reuteri JCM 1112T and its closely related species Lactobacillus fermentam IFO 3956. Both are in the same phylogenetic group within the genus Lactobacillus. Comparative genome analysis revealed that L. reuteri JCM 1112T has a unique cluster of 58 genes for the biosynthesis of reuterin and cobalamin (vitamin B12). The 58-gene cluster has a lower GC content and is apparently inserted into the conserved region, suggesting that the cluster represents a genomic island acquired from an anomalous source. Two-dimensional nuclear magnetic resonance (2D-NMR) with 13C3-glyceroI demonstrated that L. reuteri JCM 1112 T could convert glycerol to reuterin in vivo, substantiating the potential of L. reuteri JCM 1112T to produce reuterin in the intestine. Given that glycerol is shown to be naturally present in feces, the acquired ability to produce reuterin and cobalamin is an adaptive evolutionary response that likely contributes to the probiotic properties of L. reuteri.

Original languageEnglish
Pages (from-to)151-161
Number of pages11
JournalDNA Research
Volume15
Issue number3
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Lactobacillus reuteri
Lactobacillus fermentum
Genomic Islands
Lactobacillus
Vitamin B 12
Genome
Glycerol
Probiotics
Multigene Family
Anaerobiosis
Base Composition
3-hydroxypropionaldehyde
Feces
Intestines
Lactic Acid
Magnetic Resonance Spectroscopy
Bacteria

Keywords

  • Cobalamin
  • Genome
  • Lactobacillus fermentum
  • Lactobacillus reuteri
  • Reuterin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Comparative genome analysis of Lactobacillus renteri and Lactobacillus fermentum reveal a genomic Island for reuterin and cobalamin production. / Morita, Hidetoshi; Hidehiro, T. O H; Fukuda, Shinji; Horikawa, Hiroshi; Oshima, Kenshiro; Suzuki, Takehito; Murakami, Masaru; Hisamatsu, Shin; Kato, Yukio; Takizawa, Tatsuya; Fukuoka, Hideo; Yoshimura, Tetsuhiko; Itoh, Kikuji; O'Sullivan, Daniel J.; Mckay, Larry L.; Ohno, Hiroshi; Kikuchi, Jun; Masaoka, Toshio; Hattori, Masahira.

In: DNA Research, Vol. 15, No. 3, 2008, p. 151-161.

Research output: Contribution to journalArticle

Morita, H, Hidehiro, TOH, Fukuda, S, Horikawa, H, Oshima, K, Suzuki, T, Murakami, M, Hisamatsu, S, Kato, Y, Takizawa, T, Fukuoka, H, Yoshimura, T, Itoh, K, O'Sullivan, DJ, Mckay, LL, Ohno, H, Kikuchi, J, Masaoka, T & Hattori, M 2008, 'Comparative genome analysis of Lactobacillus renteri and Lactobacillus fermentum reveal a genomic Island for reuterin and cobalamin production', DNA Research, vol. 15, no. 3, pp. 151-161. https://doi.org/10.1093/dnares/dsn009
Morita, Hidetoshi ; Hidehiro, T. O H ; Fukuda, Shinji ; Horikawa, Hiroshi ; Oshima, Kenshiro ; Suzuki, Takehito ; Murakami, Masaru ; Hisamatsu, Shin ; Kato, Yukio ; Takizawa, Tatsuya ; Fukuoka, Hideo ; Yoshimura, Tetsuhiko ; Itoh, Kikuji ; O'Sullivan, Daniel J. ; Mckay, Larry L. ; Ohno, Hiroshi ; Kikuchi, Jun ; Masaoka, Toshio ; Hattori, Masahira. / Comparative genome analysis of Lactobacillus renteri and Lactobacillus fermentum reveal a genomic Island for reuterin and cobalamin production. In: DNA Research. 2008 ; Vol. 15, No. 3. pp. 151-161.
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AU - Morita, Hidetoshi

AU - Hidehiro, T. O H

AU - Fukuda, Shinji

AU - Horikawa, Hiroshi

AU - Oshima, Kenshiro

AU - Suzuki, Takehito

AU - Murakami, Masaru

AU - Hisamatsu, Shin

AU - Kato, Yukio

AU - Takizawa, Tatsuya

AU - Fukuoka, Hideo

AU - Yoshimura, Tetsuhiko

AU - Itoh, Kikuji

AU - O'Sullivan, Daniel J.

AU - Mckay, Larry L.

AU - Ohno, Hiroshi

AU - Kikuchi, Jun

AU - Masaoka, Toshio

AU - Hattori, Masahira

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N2 - Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects of L. reuteri have been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. We determined the complete genome sequences of the reuterin-producing L. reuteri JCM 1112T and its closely related species Lactobacillus fermentam IFO 3956. Both are in the same phylogenetic group within the genus Lactobacillus. Comparative genome analysis revealed that L. reuteri JCM 1112T has a unique cluster of 58 genes for the biosynthesis of reuterin and cobalamin (vitamin B12). The 58-gene cluster has a lower GC content and is apparently inserted into the conserved region, suggesting that the cluster represents a genomic island acquired from an anomalous source. Two-dimensional nuclear magnetic resonance (2D-NMR) with 13C3-glyceroI demonstrated that L. reuteri JCM 1112 T could convert glycerol to reuterin in vivo, substantiating the potential of L. reuteri JCM 1112T to produce reuterin in the intestine. Given that glycerol is shown to be naturally present in feces, the acquired ability to produce reuterin and cobalamin is an adaptive evolutionary response that likely contributes to the probiotic properties of L. reuteri.

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