Comparative genomic characterization of three streptococcus parauberis strains in fish pathogen, as assessed by wide-genome analyses

Seong Won Nho, Jun Ichi Hikima, Seong Bin Park, Ho Bin Jang, In Seok Cha, Motoshige Yasuike, Yoji Nakamura, Atsushi Fujiwara, Motohiko Sano, Kinya Kanai, Hidehiro Kondo, Ikuo Hirono, Haruko Takeyama, Takashi Aoki, Tae Sung Jung

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Streptococcus parauberis, which is the main causative agent of streptococcosis among olive flounder (Paralichthys olivaceus) in northeast Asia, can be distinctly divided into two groups (type I and type II) by an agglutination test. Here, the whole genome sequences of two Japanese strains (KRS-02083 and KRS-02109) were determined and compared with the previously determined genome of a Korean strain (KCTC 11537). The genomes of S. parauberis are intermediate in size and have lower GC contents than those of other streptococci. We annotated 2,236 and 2,048 genes in KRS-02083 and KRS-02109, respectively. Our results revealed that the three S. parauberis strains contain different genomic insertions and deletions. In particular, the genomes of Korean and Japanese strains encode different factors for sugar utilization; the former encodes the phosphotransferase system (PTS) for sorbose, whereas the latter encodes proteins for lactose hydrolysis, respectively. And the KRS-02109 strain, specifically, was the type II strain found to be able to resist phage infection through the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system and which might contribute valuably to serologically distribution. Thus, our genome-wide association study shows that polymorphisms can affect pathogen responses, providing insight into biological/biochemical pathways and phylogenetic diversity.

    Original languageEnglish
    Article numbere80395
    JournalPLoS One
    Volume8
    Issue number11
    DOIs
    Publication statusPublished - 2013 Nov 18

    Fingerprint

    Streptococcus parauberis
    Pathogens
    Streptococcus
    Fish
    Fishes
    Genes
    Genome
    genomics
    genome
    pathogens
    fish
    Clustered Regularly Interspaced Short Palindromic Repeats
    Paralichthys olivaceus
    Sorbose
    Flounder
    Agglutination Tests
    Genome-Wide Association Study
    Base Composition
    Olea
    Lactose

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Nho, S. W., Hikima, J. I., Park, S. B., Jang, H. B., Cha, I. S., Yasuike, M., ... Jung, T. S. (2013). Comparative genomic characterization of three streptococcus parauberis strains in fish pathogen, as assessed by wide-genome analyses. PLoS One, 8(11), [e80395]. https://doi.org/10.1371/journal.pone.0080395

    Comparative genomic characterization of three streptococcus parauberis strains in fish pathogen, as assessed by wide-genome analyses. / Nho, Seong Won; Hikima, Jun Ichi; Park, Seong Bin; Jang, Ho Bin; Cha, In Seok; Yasuike, Motoshige; Nakamura, Yoji; Fujiwara, Atsushi; Sano, Motohiko; Kanai, Kinya; Kondo, Hidehiro; Hirono, Ikuo; Takeyama, Haruko; Aoki, Takashi; Jung, Tae Sung.

    In: PLoS One, Vol. 8, No. 11, e80395, 18.11.2013.

    Research output: Contribution to journalArticle

    Nho, SW, Hikima, JI, Park, SB, Jang, HB, Cha, IS, Yasuike, M, Nakamura, Y, Fujiwara, A, Sano, M, Kanai, K, Kondo, H, Hirono, I, Takeyama, H, Aoki, T & Jung, TS 2013, 'Comparative genomic characterization of three streptococcus parauberis strains in fish pathogen, as assessed by wide-genome analyses', PLoS One, vol. 8, no. 11, e80395. https://doi.org/10.1371/journal.pone.0080395
    Nho, Seong Won ; Hikima, Jun Ichi ; Park, Seong Bin ; Jang, Ho Bin ; Cha, In Seok ; Yasuike, Motoshige ; Nakamura, Yoji ; Fujiwara, Atsushi ; Sano, Motohiko ; Kanai, Kinya ; Kondo, Hidehiro ; Hirono, Ikuo ; Takeyama, Haruko ; Aoki, Takashi ; Jung, Tae Sung. / Comparative genomic characterization of three streptococcus parauberis strains in fish pathogen, as assessed by wide-genome analyses. In: PLoS One. 2013 ; Vol. 8, No. 11.
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    AU - Cha, In Seok

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    AU - Kanai, Kinya

    AU - Kondo, Hidehiro

    AU - Hirono, Ikuo

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