Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes

Ken Kurokawa, Takehiko Itoh, Tomomi Kuwahara, Kenshiro Oshima, Hidehiro Toh, Atsushi Toyoda, Hideto Takami, Hidetoshi Morita, Vineet K. Sharma, Tulika P. Srivastava, Todd D. Taylor, Hideki Noguchi, Hiroshi Mori, Yoshitoshi Ogura, Dusko S. Ehrlich, Kikuji Itoh, Toshihisa Takagi, Yoshiyuki Sakaki, Tetsuya Hayashi, Masahira Hattori

Research output: Contribution to journalArticle

561 Citations (Scopus)

Abstract

Numerous microbes inhabit the human intestine, many of which are uncharacterized or uncultivable. They form a complex microbial community that deeply affects human physiology. To identify the genomic features common to all human gut microbiomes as well as those variable among them, we performed a large-scale comparative metagenomic analysis of fecal samples from 13 healthy individuals of various ages, including unweaned infants. We found that, while the gut microbiota from unweaned infants were simple and showed a high inter-individual variation in taxonomic and gene composition, those from adults and weaned children were more complex but showed a high functional uniformity regardless of age or sex. In searching for the genes over-represented in gut microbiomes, we identified 237 gene families commonly enriched in adult-type and 136 families in infant-type microbiomes, with a small overlap. An analysis of their predicted functions revealed various strategies employed by each type of microbiota to adapt to its intestinal environment, suggesting that these gene sets encode the core functions of adult and infant-type gut microbiota. By analysing the orphan genes, 647 new gene families were identified to be exclusively present in human intestinal microbiomes. In addition, we discovered a conjugative transposon family explosively amplified in human gut microbiomes, which strongly suggests that the intestine is a 'hot spot' for horizontal gene transfer between microbes.

Original languageEnglish
Pages (from-to)169-181
Number of pages13
JournalDNA Research
Volume14
Issue number4
DOIs
Publication statusPublished - 2007 Sep
Externally publishedYes

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Metagenomics
Microbiota
Genes
Intestines
Horizontal Gene Transfer
Orphaned Children
Gastrointestinal Microbiome

Keywords

  • Conjugative transposon
  • Gene family
  • Human gut microbiota
  • Metagenomics

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes. / Kurokawa, Ken; Itoh, Takehiko; Kuwahara, Tomomi; Oshima, Kenshiro; Toh, Hidehiro; Toyoda, Atsushi; Takami, Hideto; Morita, Hidetoshi; Sharma, Vineet K.; Srivastava, Tulika P.; Taylor, Todd D.; Noguchi, Hideki; Mori, Hiroshi; Ogura, Yoshitoshi; Ehrlich, Dusko S.; Itoh, Kikuji; Takagi, Toshihisa; Sakaki, Yoshiyuki; Hayashi, Tetsuya; Hattori, Masahira.

In: DNA Research, Vol. 14, No. 4, 09.2007, p. 169-181.

Research output: Contribution to journalArticle

Kurokawa, K, Itoh, T, Kuwahara, T, Oshima, K, Toh, H, Toyoda, A, Takami, H, Morita, H, Sharma, VK, Srivastava, TP, Taylor, TD, Noguchi, H, Mori, H, Ogura, Y, Ehrlich, DS, Itoh, K, Takagi, T, Sakaki, Y, Hayashi, T & Hattori, M 2007, 'Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes', DNA Research, vol. 14, no. 4, pp. 169-181. https://doi.org/10.1093/dnares/dsm018
Kurokawa K, Itoh T, Kuwahara T, Oshima K, Toh H, Toyoda A et al. Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes. DNA Research. 2007 Sep;14(4):169-181. https://doi.org/10.1093/dnares/dsm018
Kurokawa, Ken ; Itoh, Takehiko ; Kuwahara, Tomomi ; Oshima, Kenshiro ; Toh, Hidehiro ; Toyoda, Atsushi ; Takami, Hideto ; Morita, Hidetoshi ; Sharma, Vineet K. ; Srivastava, Tulika P. ; Taylor, Todd D. ; Noguchi, Hideki ; Mori, Hiroshi ; Ogura, Yoshitoshi ; Ehrlich, Dusko S. ; Itoh, Kikuji ; Takagi, Toshihisa ; Sakaki, Yoshiyuki ; Hayashi, Tetsuya ; Hattori, Masahira. / Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes. In: DNA Research. 2007 ; Vol. 14, No. 4. pp. 169-181.
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