Comparative phylogenetic analyses of Halomonas variabilis and related organisms based on 16S rRNA, gyrB and ectBC gene sequences

Takuji Okamoto, Akihiko Maruyama, Satoshi Imura, Haruko Takeyama, Takeshi Naganuma

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Halomonas variabilis and phylogenetically related organisms were isolated from various habitats such as Antarctic terrain and saline ponds, deep-sea sediment, deep-sea waters affected by hydrothermal plumes, and hydrothermal vent fluids. Ten strains were selected for physiological and phylogenetic characterization in detail. All of those strains were found to be piezotolerant and psychrotolerant, as well as euryhaline halophilic or halotolerant. Their stress tolerance may facilitate their wide occurrence, even in so-called extreme environments. The 16S rDNA-based phylogenetic relationship was complemented by analyses of the DNA gyrase subunit B gene (gyrB) and genes involved in the synthesis of the major compatible solute, ectoine: diaminobutyric acid aminotransferase gene (ectB) and ectoine synthase gene (ectC). The phylogenetic relationships of H. variabilis and related organisms were very similar in terms of 16S rDNA, gyrB, and ectB. The ectC-based tree was inconsistent with the other phylogenetic trees. For that reason, ectC was inferred to derive from horizontal transfer.

Original languageEnglish
Pages (from-to)323-333
Number of pages11
JournalSystematic and Applied Microbiology
Volume27
Issue number3
DOIs
Publication statusPublished - 2004 May
Externally publishedYes

Fingerprint

Halomonas variabilis
Halomonas
ribosomal RNA
phylogenetics
nucleotide sequences
gene
phylogeny
organisms
Ribosomal DNA
Genes
genes
Hydrothermal Vents
DNA Gyrase
hydrothermal plume
DNA topoisomerase (ATP-hydrolysing)
deep-sea sediment
hydrothermal vent
Seawater
transaminases
Transaminases

Keywords

  • 16S rDNA
  • Cosmopolitan
  • ectBC
  • Euryhaline halophile
  • gyrB
  • Halomonas variabilis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Comparative phylogenetic analyses of Halomonas variabilis and related organisms based on 16S rRNA, gyrB and ectBC gene sequences. / Okamoto, Takuji; Maruyama, Akihiko; Imura, Satoshi; Takeyama, Haruko; Naganuma, Takeshi.

In: Systematic and Applied Microbiology, Vol. 27, No. 3, 05.2004, p. 323-333.

Research output: Contribution to journalArticle

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