Comparative sequence analysis of a multidrug-resistant plasmid from Aeromonas hydrophila

Carmelo S. Del Castillo, Jun Ichi Hikima, Ho Bin Jang, Seong Won Nho, Tae Sung Jung, Janenuj Wongtavatchai, Hidehiro Kondo, Ikuo Hirono, Haruko Takeyama, Takashi Aokia

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    Aeromonas hydrophila is a pathogenic bacterium that has been implicated in fish, animal, and human disease. Recently, a multidrug resistance (MDR) plasmid, pR148, was isolated from A. hydrophila obtained from a tilapia (Oreochromis niloticus) farm in Thailand. pR148 is a 165,906-bp circular plasmid containing 147 coding regions showing highest similarity to pNDM-1-Dok1, an MDR plasmid isolated from a human pathogen. pR148 was also very similar to other IncA/C plasmids isolated from humans, animals, food, and fish. pR148 contains a mercuric resistance operon and encodes the complete set of genes for the type 4 secretion system. pR148 encodes a Tn21 type transposon. This transposon contains the drug resistance genes qacH, blaOXA-10, aadA1, and sul1 in a class 1 integron; tetA and tetR in transposon Tn1721; and catA2 and a duplicate sul1 in a locus showing 100% similarity to IncU plasmids isolated from fish. The blaOXA-10 and aadA1 genes showed 100% similarity to those from the Acinetobacter baumannii AYE genome. The similarity of pR148 to a human pathogen-derived plasmid indicates that the plasmids were either transferred between different genera or that they are derived from a common origin. Previous studies have shown that IncA/C plasmids retain a conserved backbone, while the accessory region points to lateral gene transfer. These observations point out the dangers of indiscriminate use of antibiotics in humans and in animals and the necessity of understanding how drug resistance determinants are disseminated and transferred.

    Original languageEnglish
    Pages (from-to)120-129
    Number of pages10
    JournalAntimicrobial Agents and Chemotherapy
    Volume57
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jan

    Fingerprint

    Aeromonas hydrophila
    Sequence Analysis
    Plasmids
    Fishes
    Multiple Drug Resistance
    Drug Resistance
    Integrons
    Genes
    Tilapia
    Cichlids
    Acinetobacter baumannii
    Horizontal Gene Transfer
    Animal Diseases
    Thailand
    Operon
    Genome
    Anti-Bacterial Agents
    Bacteria
    Food

    ASJC Scopus subject areas

    • Pharmacology (medical)
    • Pharmacology
    • Infectious Diseases

    Cite this

    Del Castillo, C. S., Hikima, J. I., Jang, H. B., Nho, S. W., Jung, T. S., Wongtavatchai, J., ... Aokia, T. (2013). Comparative sequence analysis of a multidrug-resistant plasmid from Aeromonas hydrophila. Antimicrobial Agents and Chemotherapy, 57(1), 120-129. https://doi.org/10.1128/AAC.01239-12

    Comparative sequence analysis of a multidrug-resistant plasmid from Aeromonas hydrophila. / Del Castillo, Carmelo S.; Hikima, Jun Ichi; Jang, Ho Bin; Nho, Seong Won; Jung, Tae Sung; Wongtavatchai, Janenuj; Kondo, Hidehiro; Hirono, Ikuo; Takeyama, Haruko; Aokia, Takashi.

    In: Antimicrobial Agents and Chemotherapy, Vol. 57, No. 1, 01.2013, p. 120-129.

    Research output: Contribution to journalArticle

    Del Castillo, CS, Hikima, JI, Jang, HB, Nho, SW, Jung, TS, Wongtavatchai, J, Kondo, H, Hirono, I, Takeyama, H & Aokia, T 2013, 'Comparative sequence analysis of a multidrug-resistant plasmid from Aeromonas hydrophila', Antimicrobial Agents and Chemotherapy, vol. 57, no. 1, pp. 120-129. https://doi.org/10.1128/AAC.01239-12
    Del Castillo, Carmelo S. ; Hikima, Jun Ichi ; Jang, Ho Bin ; Nho, Seong Won ; Jung, Tae Sung ; Wongtavatchai, Janenuj ; Kondo, Hidehiro ; Hirono, Ikuo ; Takeyama, Haruko ; Aokia, Takashi. / Comparative sequence analysis of a multidrug-resistant plasmid from Aeromonas hydrophila. In: Antimicrobial Agents and Chemotherapy. 2013 ; Vol. 57, No. 1. pp. 120-129.
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    abstract = "Aeromonas hydrophila is a pathogenic bacterium that has been implicated in fish, animal, and human disease. Recently, a multidrug resistance (MDR) plasmid, pR148, was isolated from A. hydrophila obtained from a tilapia (Oreochromis niloticus) farm in Thailand. pR148 is a 165,906-bp circular plasmid containing 147 coding regions showing highest similarity to pNDM-1-Dok1, an MDR plasmid isolated from a human pathogen. pR148 was also very similar to other IncA/C plasmids isolated from humans, animals, food, and fish. pR148 contains a mercuric resistance operon and encodes the complete set of genes for the type 4 secretion system. pR148 encodes a Tn21 type transposon. This transposon contains the drug resistance genes qacH, blaOXA-10, aadA1, and sul1 in a class 1 integron; tetA and tetR in transposon Tn1721; and catA2 and a duplicate sul1 in a locus showing 100{\%} similarity to IncU plasmids isolated from fish. The blaOXA-10 and aadA1 genes showed 100{\%} similarity to those from the Acinetobacter baumannii AYE genome. The similarity of pR148 to a human pathogen-derived plasmid indicates that the plasmids were either transferred between different genera or that they are derived from a common origin. Previous studies have shown that IncA/C plasmids retain a conserved backbone, while the accessory region points to lateral gene transfer. These observations point out the dangers of indiscriminate use of antibiotics in humans and in animals and the necessity of understanding how drug resistance determinants are disseminated and transferred.",
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