Abstract
Two ribozymes, hammerhead ribozyme and hairpin ribozyme, and a DNA-enzyme were designed to cleave a same RNA target, the same site of the rat complement regulatory factor 512 antigen mRNA. The kinetic properties of these RNA-cleaving enzymes were measured and compared under the same conditions, using multiple turnover kinetics and competition kinetics. The catalytic efficiencies of these enzymes, and also the order of these enzymes will be discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 217-218 |
| Number of pages | 2 |
| Journal | Nucleic acids symposium series |
| Issue number | 42 |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Comparative study of two ribozymes and DNA-enzyme against the same RNA target. / Inui, O.; Kikuchi, Yo; Dohi, N.; Okada, N.; Okada, H.; Tanaka, T.
In: Nucleic acids symposium series, No. 42, 1999, p. 217-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative study of two ribozymes and DNA-enzyme against the same RNA target.
AU - Inui, O.
AU - Kikuchi, Yo
AU - Dohi, N.
AU - Okada, N.
AU - Okada, H.
AU - Tanaka, T.
PY - 1999
Y1 - 1999
N2 - Two ribozymes, hammerhead ribozyme and hairpin ribozyme, and a DNA-enzyme were designed to cleave a same RNA target, the same site of the rat complement regulatory factor 512 antigen mRNA. The kinetic properties of these RNA-cleaving enzymes were measured and compared under the same conditions, using multiple turnover kinetics and competition kinetics. The catalytic efficiencies of these enzymes, and also the order of these enzymes will be discussed.
AB - Two ribozymes, hammerhead ribozyme and hairpin ribozyme, and a DNA-enzyme were designed to cleave a same RNA target, the same site of the rat complement regulatory factor 512 antigen mRNA. The kinetic properties of these RNA-cleaving enzymes were measured and compared under the same conditions, using multiple turnover kinetics and competition kinetics. The catalytic efficiencies of these enzymes, and also the order of these enzymes will be discussed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033289571&partnerID=8YFLogxK
M3 - Article
C2 - 10780457
AN - SCOPUS:0033289571
SP - 217
EP - 218
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
SN - 0261-3166
IS - 42
ER -