Comparison between the CENP-A and histone H3 structures in nucleosomes

Hiroaki Tachiwana, Wataru Kagawa, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.

    Original languageEnglish
    Pages (from-to)6-11
    Number of pages6
    JournalNucleus
    Volume3
    Issue number1
    DOIs
    Publication statusPublished - 2012 Jan

    Fingerprint

    Nucleosomes
    Histones
    Centromere
    centromere protein A
    DNA
    Base Pairing

    Keywords

    • CENP-A
    • CENP-B
    • CENP-C
    • Centromere
    • Chromatin
    • Histone
    • Histone H3
    • Nucleosome

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Comparison between the CENP-A and histone H3 structures in nucleosomes. / Tachiwana, Hiroaki; Kagawa, Wataru; Kurumizaka, Hitoshi.

    In: Nucleus, Vol. 3, No. 1, 01.2012, p. 6-11.

    Research output: Contribution to journalArticle

    Tachiwana, H, Kagawa, W & Kurumizaka, H 2012, 'Comparison between the CENP-A and histone H3 structures in nucleosomes', Nucleus, vol. 3, no. 1, pp. 6-11. https://doi.org/10.4161/nucl.3.1.18372
    Tachiwana, Hiroaki ; Kagawa, Wataru ; Kurumizaka, Hitoshi. / Comparison between the CENP-A and histone H3 structures in nucleosomes. In: Nucleus. 2012 ; Vol. 3, No. 1. pp. 6-11.
    @article{b041dd5895484e68aba5a10dbc3379bd,
    title = "Comparison between the CENP-A and histone H3 structures in nucleosomes",
    abstract = "Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.",
    keywords = "CENP-A, CENP-B, CENP-C, Centromere, Chromatin, Histone, Histone H3, Nucleosome",
    author = "Hiroaki Tachiwana and Wataru Kagawa and Hitoshi Kurumizaka",
    year = "2012",
    month = "1",
    doi = "10.4161/nucl.3.1.18372",
    language = "English",
    volume = "3",
    pages = "6--11",
    journal = "Nucleus",
    issn = "0115-2300",
    publisher = "Landes Bioscience",
    number = "1",

    }

    TY - JOUR

    T1 - Comparison between the CENP-A and histone H3 structures in nucleosomes

    AU - Tachiwana, Hiroaki

    AU - Kagawa, Wataru

    AU - Kurumizaka, Hitoshi

    PY - 2012/1

    Y1 - 2012/1

    N2 - Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.

    AB - Centromeres are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A (CENP-A nucleosome) and their inheritance is probably dictated by the architecture of the centromeric nucleosome. We previously determined the crystal structure of the human CENP-A nucleosome. CENP-A forms a histone octamer containing two each of histones H2A, H2B, H4 and CENP-A and the DNA is left-handedly wrapped around the histone octamer, as in canonical nucleosomes containing histone H3. In the CENP-A nucleosome structure, 13 base pairs of the DNA ends are detached from the histone surface and two CENP-A regions, the aN helix and loop 1, adopt different structures from those in the H3 nucleosome. In this Extra View article, we provide a detailed structural comparison between CENP-A and H3 in nucleosomes and describe their distinctions and similarities.

    KW - CENP-A

    KW - CENP-B

    KW - CENP-C

    KW - Centromere

    KW - Chromatin

    KW - Histone

    KW - Histone H3

    KW - Nucleosome

    UR - http://www.scopus.com/inward/record.url?scp=84859138416&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84859138416&partnerID=8YFLogxK

    U2 - 10.4161/nucl.3.1.18372

    DO - 10.4161/nucl.3.1.18372

    M3 - Article

    C2 - 22127263

    AN - SCOPUS:84859138416

    VL - 3

    SP - 6

    EP - 11

    JO - Nucleus

    JF - Nucleus

    SN - 0115-2300

    IS - 1

    ER -