Comparison of Ca2+ uptake characteristics of microsomal fractions isolated from unfertilized and fertilized sea urchin eggs

H. Inoue, T. Yoshioka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The microsomal fraction isolated from sea urchin H. pulcherrimus eggs has the ability to actively accumulate Ca2+ in the presence of ATP. The Ca2+ uptake was sustained by addition of oxalate and was apparently insensitive to sodium azide. The sequestered microsomal Ca was readily released by the divalent cation ionophore A23187. The microsomal fraction obtained from fertilized eggs accumulated Ca2+ about five times more quickly than did that from unfertilized eggs. The increased Ca2+ uptake by microsomal fraction obtained from fertilized eggs was due to an increase in the maximum velocity of Ca2+ uptake and there was no difference in Km for calcium between the two fractions.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalExperimental Cell Research
Volume140
Issue number2
DOIs
Publication statusPublished - 1982
Externally publishedYes

Fingerprint

Sea Urchins
Zygote
Eggs
Sodium Azide
Oxalates
Ionophores
Divalent Cations
Calcimycin
Ovum
Adenosine Triphosphate
Calcium

ASJC Scopus subject areas

  • Cell Biology

Cite this

Comparison of Ca2+ uptake characteristics of microsomal fractions isolated from unfertilized and fertilized sea urchin eggs. / Inoue, H.; Yoshioka, T.

In: Experimental Cell Research, Vol. 140, No. 2, 1982, p. 283-288.

Research output: Contribution to journalArticle

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