Comparison of catalase in diploid and haploid Rana rugosa using heat and chemical inactivation techniques

Akihiko Kashiwagi, Keiko Kashiwagi, Minoru Takase, Hideki Hanada, Masahisa Nakamura

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The present study examines differences in the hydrogen peroxide (H2O2) detoxifying enzyme, catalase, found in the tails and livers of diploid and haploid Rana rugosa. Investigative techniques include measurement of catalase activity and tests for temperature stability and chemical inhibition. Catalase from the tails of pre-climactic (stage XXIII) haploids was found to be over three times as H2O2 destructive as catalase from similar tails of diploids. Catalase from the livers of newly metamorphosed (stage XXV) froglets, on the other hand, displayed only one third the activity seen in diploid livers. The catalase in haploid tail and liver proved to be more heat resistant, retaining 40-60% of its original activity after 5 min of treatment at 55°C, whereas diploid catalase was totally inactivated under the same conditions. Haploid and diploid catalase also responded differently to inhibition using urea and aminotriazole. These differences suggest that haploid catalase has diverged from normal diploid catalase through molecular modification, resulting in abnormal systems for H2O2 metabolism, which in turn are thought to be responsible for organ dysfunction and early death seen in haploid individuals.

Original languageEnglish
Pages (from-to)499-503
Number of pages5
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume118
Issue number3
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Ranidae
Haploidy
Diploidy
Catalase
Hot Temperature
Liver
Tail
Investigative Techniques
Amitrole
Metabolism
Hydrogen Peroxide
Urea

Keywords

  • Anuran haploids
  • Apoptosis
  • Catalase differences
  • Catalase temperature stability
  • Excess HO removal
  • Hydrogen peroxide metabolism
  • Poor haploid viability
  • Programmed cell death
  • Urea and aminotriazole inhibition of catalase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

Comparison of catalase in diploid and haploid Rana rugosa using heat and chemical inactivation techniques. / Kashiwagi, Akihiko; Kashiwagi, Keiko; Takase, Minoru; Hanada, Hideki; Nakamura, Masahisa.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 118, No. 3, 1997, p. 499-503.

Research output: Contribution to journalArticle

Kashiwagi, Akihiko ; Kashiwagi, Keiko ; Takase, Minoru ; Hanada, Hideki ; Nakamura, Masahisa. / Comparison of catalase in diploid and haploid Rana rugosa using heat and chemical inactivation techniques. In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 1997 ; Vol. 118, No. 3. pp. 499-503.
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