Comparison of detection specificity of nitrifying bacteria in biofilm using fluorescence in situ hybridization and in situ fluorescent antibody methods

N. Noda, Y. Ebie, M. Matsumura, S. Tsuneda, A. Hirata, Y. Inamori

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The in situ fluorescent antibody and fluorescence in situ hybridization (FISH) methods are very useful in the in situ detection of specific bacteria like nitrifiers in a biofilm. In this study, simultaneous staining using the FISH and in situ fluorescent antibody methods was examined. As a result, no specific fluorescence was observed with either method when FISH was performed followed by the in situ fluorescent antibody method; however, when the in situ fluorescent antibody method was performed first followed by FISH, specific fluorescence was observed in both cases. Moreover, it was suggested that the detection specificities of FISH and the in situ fluorescent antibody method are almost identical.

Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalWater Science and Technology
Volume47
Issue number5
Publication statusPublished - 2003 May 6

Keywords

  • Fluorescence in situ hybridization
  • Fluorescent antibody method
  • Monoclonal antibody
  • Nitrifying bacteria

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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