Comparison of detection specificity of nitrifying bacteria in biofilm using fluorescence in situ hybridization and in situ fluorescent antibody methods

N. Noda, Y. Ebie, M. Matsumura, Satoshi Tsuneda, A. Hirata, Y. Inamori

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    The in situ fluorescent antibody and fluorescence in situ hybridization (FISH) methods are very useful in the in situ detection of specific bacteria like nitrifiers in a biofilm. In this study, simultaneous staining using the FISH and in situ fluorescent antibody methods was examined. As a result, no specific fluorescence was observed with either method when FISH was performed followed by the in situ fluorescent antibody method; however, when the in situ fluorescent antibody method was performed first followed by FISH, specific fluorescence was observed in both cases. Moreover, it was suggested that the detection specificities of FISH and the in situ fluorescent antibody method are almost identical.

    Original languageEnglish
    Pages (from-to)129-132
    Number of pages4
    JournalWater Science and Technology
    Volume47
    Issue number5
    Publication statusPublished - 2003

    Fingerprint

    nitrifying bacterium
    Biofilms
    Antibodies
    antibody
    biofilm
    Bacteria
    fluorescence
    Fluorescence
    method
    detection
    comparison
    in situ

    Keywords

    • Fluorescence in situ hybridization
    • Fluorescent antibody method
    • Monoclonal antibody
    • Nitrifying bacteria

    ASJC Scopus subject areas

    • Water Science and Technology

    Cite this

    Comparison of detection specificity of nitrifying bacteria in biofilm using fluorescence in situ hybridization and in situ fluorescent antibody methods. / Noda, N.; Ebie, Y.; Matsumura, M.; Tsuneda, Satoshi; Hirata, A.; Inamori, Y.

    In: Water Science and Technology, Vol. 47, No. 5, 2003, p. 129-132.

    Research output: Contribution to journalArticle

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    AU - Ebie, Y.

    AU - Matsumura, M.

    AU - Tsuneda, Satoshi

    AU - Hirata, A.

    AU - Inamori, Y.

    PY - 2003

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    KW - Fluorescent antibody method

    KW - Monoclonal antibody

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