Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA

Mutsunori Shirai, Hideki Hirakawa, Mitsuaki Kimoto, Mitsuaki Tabuchi, Fumio Kishi, Kazunobu Ouchi, Tadayoshi Shiba, Kazuo Ishii, Masahira Hattori, Satoru Kuhara, Teruko Nakazawa

Research output: Contribution to journalArticle

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Abstract

Chlamydia pneumoniae is a widespread pathogen of humans causing pneumonia and bronchitis. There are many reports of an association between C. pneumoniae infection and atherosclerosis. We determined the whole genome sequence of C. pneumoniae strain J138 isolated in Japan in 1994 and compared it with the sequence of strain CWL029 isolated in the USA before 1987. The J138 circular chromosome consists of 1,226,565 nt (40.7% G + C) with 1072 likely protein-coding genes that is 3665 nt shorter than the CWL029 genome. Plasmids, phage- or transposon-like sequences were not identified. The overall genomic organization, gene order and predicted proteomes of the two strains are very similar, suggesting a high level of structural and functional conservation between the two unrelated isolates. The most conspicuous differences in the J138 genome relative to the CWL029 genome are the absence of five DNA segments, ranging in size from 89 to 1649 nt, and the presence of three DNA segments, ranging from 27 to 84 nt. The complex organization of these 'different zones' may be attributable to a unique system of recombination.

Original languageEnglish
Pages (from-to)2311-2314
Number of pages4
JournalNucleic Acids Research
Volume28
Issue number12
Publication statusPublished - 2000 Jun 15
Externally publishedYes

Fingerprint

Chlamydophila pneumoniae
Japan
Genome
Chlamydia Infections
Gene Order
Bronchitis
Chromosomes, Human, Pair 1
DNA
Proteome
Bacteriophages
Genetic Recombination
Atherosclerosis
Pneumonia
Plasmids
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Shirai, M., Hirakawa, H., Kimoto, M., Tabuchi, M., Kishi, F., Ouchi, K., ... Nakazawa, T. (2000). Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA. Nucleic Acids Research, 28(12), 2311-2314.

Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA. / Shirai, Mutsunori; Hirakawa, Hideki; Kimoto, Mitsuaki; Tabuchi, Mitsuaki; Kishi, Fumio; Ouchi, Kazunobu; Shiba, Tadayoshi; Ishii, Kazuo; Hattori, Masahira; Kuhara, Satoru; Nakazawa, Teruko.

In: Nucleic Acids Research, Vol. 28, No. 12, 15.06.2000, p. 2311-2314.

Research output: Contribution to journalArticle

Shirai, M, Hirakawa, H, Kimoto, M, Tabuchi, M, Kishi, F, Ouchi, K, Shiba, T, Ishii, K, Hattori, M, Kuhara, S & Nakazawa, T 2000, 'Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA', Nucleic Acids Research, vol. 28, no. 12, pp. 2311-2314.
Shirai M, Hirakawa H, Kimoto M, Tabuchi M, Kishi F, Ouchi K et al. Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA. Nucleic Acids Research. 2000 Jun 15;28(12):2311-2314.
Shirai, Mutsunori ; Hirakawa, Hideki ; Kimoto, Mitsuaki ; Tabuchi, Mitsuaki ; Kishi, Fumio ; Ouchi, Kazunobu ; Shiba, Tadayoshi ; Ishii, Kazuo ; Hattori, Masahira ; Kuhara, Satoru ; Nakazawa, Teruko. / Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA. In: Nucleic Acids Research. 2000 ; Vol. 28, No. 12. pp. 2311-2314.
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