Complete deoxygenation from a Hemoglobin solution by an electrochemical method and heat treatment for virus inactivation

Yubin Huang, Shinji Takeoka, Shinji Takeoka, Hideki Abe, Junichi Hirayama, Kenji Ikebuchi, Hisami Ikeda, Eishun Tsuchida

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Hemoglobin (Hb) has been widely studied as a raw material for various types of oxygen carriers. In the purification of Hb from red blood cells including virus inactivation and denaturation of other proteins and the long-term storage of Hb vesicles (HbV), a deoxygenation process is one of the important processes because of the high stability of deoxygenated Hb to heating and metHb formation. Though an oxygenated Hb solution can be deoxygenated with an artificial lung, it is difficult to reduce the oxygen partial pressure of the Hb solution to less than 10 Torr. We developed an electrochemical system for complete deoxygenation of the Hb solution at the cathode compartment using hydrogen containing nitrogen gas at the anode compartment. Oxygen in the Hb solution was reduced to OH- at the cathode compartment within several minutes at a potential value of-1.67 V and was finally converted to water by neutralization with H+ from the anode in the whole system. The resulting completely deoxygenated Hb could tolerate heat treatment at 62 °C for 10 h with no denaturation of deoxygenated Hb. The metHb formation rate of reoxygenated Hb at 37 °C was not changed after heat treatment. Furthermore, vesicular stomatitis virus (VSV) could be inactivated at an inactivation degree of more than 5.96 log by heat treatment.

    Original languageEnglish
    Pages (from-to)101-107
    Number of pages7
    JournalBiotechnology Progress
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Virus Inactivation
    hemoglobin
    inactivation
    Hemoglobins
    Hot Temperature
    heat treatment
    viruses
    Electrodes
    methodology
    Oxygen
    oxygen
    Vesiculovirus
    Protein Denaturation
    Vesicular Stomatitis
    Partial Pressure
    denaturation
    neutralization
    Heating
    hydrogen
    raw materials

    ASJC Scopus subject areas

    • Food Science
    • Biotechnology
    • Microbiology

    Cite this

    Complete deoxygenation from a Hemoglobin solution by an electrochemical method and heat treatment for virus inactivation. / Huang, Yubin; Takeoka, Shinji; Takeoka, Shinji; Abe, Hideki; Hirayama, Junichi; Ikebuchi, Kenji; Ikeda, Hisami; Tsuchida, Eishun.

    In: Biotechnology Progress, Vol. 18, No. 1, 2002, p. 101-107.

    Research output: Contribution to journalArticle

    Huang, Yubin ; Takeoka, Shinji ; Takeoka, Shinji ; Abe, Hideki ; Hirayama, Junichi ; Ikebuchi, Kenji ; Ikeda, Hisami ; Tsuchida, Eishun. / Complete deoxygenation from a Hemoglobin solution by an electrochemical method and heat treatment for virus inactivation. In: Biotechnology Progress. 2002 ; Vol. 18, No. 1. pp. 101-107.
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    AU - Takeoka, Shinji

    AU - Takeoka, Shinji

    AU - Abe, Hideki

    AU - Hirayama, Junichi

    AU - Ikebuchi, Kenji

    AU - Ikeda, Hisami

    AU - Tsuchida, Eishun

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