Complete genome sequence and comparative analysis of the fish pathogen lactococcus garvieae

Hidetoshi Morita, Hidehiro Toh, Kenshiro Oshima, Mariko Yoshizaki, Michiko Kawanishi, Kohei Nakaya, Takehito Suzuki, Eiji Miyauchi, Yasuo Ishii, Soichi Tanabe, Masaru Murakami, Masahira Hattori

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Lactococcus garvieae causes fatal haemorrhagic septicaemia in fish such as yellowtail. The comparative analysis of genomes of a virulent strain Lg2 and a non-virulent strain ATCC 49156 of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsule gene cluster that is absent in ATCC 49156. The capsule gene cluster was composed of 15 genes, of which eight genes are highly conserved with those in exopolysaccharide biosynthesis gene cluster often found in Lactococcus lactis strains. Sequence analysis of the capsule gene cluster in the less virulent strain L. garvieae Lg2-S, Lg2-derived strain, showed that two conserved genes were disrupted by a single base pair deletion, respectively. These results strongly suggest that the capsule is crucial for virulence of Lg2. The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes. The analysis also predicted other potential virulence factors such as haemolysin. The present study provides new insights into understanding of the virulence mechanisms of L. garvieae in fish.

Original languageEnglish
Article numbere23184
JournalPLoS One
Volume6
Issue number8
DOIs
Publication statusPublished - 2011
Externally publishedYes

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Lactococcus
Lactococcus garvieae
Pathogens
Multigene Family
multigene family
Fish
Capsules
Sequence Analysis
Fishes
Genes
Genome
genome
pathogens
fish
virulence
Virulence
Hemorrhagic Septicemia
genomic islands
Genomic Islands
Lactococcus lactis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Complete genome sequence and comparative analysis of the fish pathogen lactococcus garvieae. / Morita, Hidetoshi; Toh, Hidehiro; Oshima, Kenshiro; Yoshizaki, Mariko; Kawanishi, Michiko; Nakaya, Kohei; Suzuki, Takehito; Miyauchi, Eiji; Ishii, Yasuo; Tanabe, Soichi; Murakami, Masaru; Hattori, Masahira.

In: PLoS One, Vol. 6, No. 8, e23184, 2011.

Research output: Contribution to journalArticle

Morita, H, Toh, H, Oshima, K, Yoshizaki, M, Kawanishi, M, Nakaya, K, Suzuki, T, Miyauchi, E, Ishii, Y, Tanabe, S, Murakami, M & Hattori, M 2011, 'Complete genome sequence and comparative analysis of the fish pathogen lactococcus garvieae', PLoS One, vol. 6, no. 8, e23184. https://doi.org/10.1371/journal.pone.0023184
Morita, Hidetoshi ; Toh, Hidehiro ; Oshima, Kenshiro ; Yoshizaki, Mariko ; Kawanishi, Michiko ; Nakaya, Kohei ; Suzuki, Takehito ; Miyauchi, Eiji ; Ishii, Yasuo ; Tanabe, Soichi ; Murakami, Masaru ; Hattori, Masahira. / Complete genome sequence and comparative analysis of the fish pathogen lactococcus garvieae. In: PLoS One. 2011 ; Vol. 6, No. 8.
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