Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis

Haruo Ikeda, Jun Ishikawa, Akiharu Hanamoto, Mayumi Shinose, Hisashi Kikuchi, Tadayoshi Shiba, Yoshiyuki Sakaki, Masahira Hattori, Satoshi Omura

Research output: Contribution to journalArticle

880 Citations (Scopus)

Abstract

Species of the genus Streptomyces are of major pharmaceutical interest because they synthesize a variety of bioactive secondary metabolites. We have determined the complete nucleotide sequence of the linear chromosome of Streptomyces avermitilis. S. avermitilis produces avermectins, a group of antiparasitic agents used in human and veterinary medicine. The genome contains 9,025,608 bases (average GC content, 70.7%) and encodes at least 7,574 potential open reading frames (ORFs). Thirty-five percent of the ORFs (2,664) constitute 721 paralogous families. Thirty gene clusters related to secondary metabolite biosynthesis were identified, corresponding to 6.6% of the genome. Comparison with Streptomyces coelicolor A3(2) revealed that an internal 6.5-Mb region in the S. avermitilis genome was highly conserved with respect to gene order and content, and contained all known essential genes but showed perfectly asymmetric structure at the oriC center. In contrast, the terminal regions were not conserved and preferentially contained nonessential genes.

Original languageEnglish
Pages (from-to)526-531
Number of pages6
JournalNature Biotechnology
Volume21
Issue number5
DOIs
Publication statusPublished - 2003 May 1
Externally publishedYes

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Streptomyces
Microorganisms
Sequence Analysis
Genes
Genome
Open Reading Frames
Streptomyces coelicolor
Antiparasitic Agents
Gene Order
Veterinary Medicine
Essential Genes
Base Composition
Multigene Family
Metabolites
Chromosomes
Veterinary medicine
Biosynthesis
Pharmaceutical Preparations
Nucleotides
Drug products

ASJC Scopus subject areas

  • Microbiology

Cite this

Ikeda, H., Ishikawa, J., Hanamoto, A., Shinose, M., Kikuchi, H., Shiba, T., ... Omura, S. (2003). Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nature Biotechnology, 21(5), 526-531. https://doi.org/10.1038/nbt820

Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. / Ikeda, Haruo; Ishikawa, Jun; Hanamoto, Akiharu; Shinose, Mayumi; Kikuchi, Hisashi; Shiba, Tadayoshi; Sakaki, Yoshiyuki; Hattori, Masahira; Omura, Satoshi.

In: Nature Biotechnology, Vol. 21, No. 5, 01.05.2003, p. 526-531.

Research output: Contribution to journalArticle

Ikeda, H, Ishikawa, J, Hanamoto, A, Shinose, M, Kikuchi, H, Shiba, T, Sakaki, Y, Hattori, M & Omura, S 2003, 'Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis', Nature Biotechnology, vol. 21, no. 5, pp. 526-531. https://doi.org/10.1038/nbt820
Ikeda, Haruo ; Ishikawa, Jun ; Hanamoto, Akiharu ; Shinose, Mayumi ; Kikuchi, Hisashi ; Shiba, Tadayoshi ; Sakaki, Yoshiyuki ; Hattori, Masahira ; Omura, Satoshi. / Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. In: Nature Biotechnology. 2003 ; Vol. 21, No. 5. pp. 526-531.
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