Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater

Tohru Shimizu, Kaori Ohtani, Hideki Hirakawa, Kenshiro Ohshima, Atsushi Yamashita, Tadayoshi Shiba, Naotake Ogasawara, Masahira Hattori, Satoru Kuhara, Hideo Hayashi

Research output: Contribution to journalArticle

497 Citations (Scopus)

Abstract

Clostridium perfringens is a Gram-positive anaerobic spore-forming bacterium that causes life-threatening gas gangrene and mild enterotoxaemia in humans, although it colonizes as normal intestinal flora of humans and animals. The organism is known to produce a variety of toxins and enzymes that are responsible for the severe myonecrotic lesions. Here we report the complete 3,031,430-bp sequence of C. perfringens strain 13 that comprises 2,660 protein coding regions and 10 rRNA genes, showing pronounced low overall G + C content (28.6%). The genome contains typical anaerobic fermentation enzymes leading to gas production but no enzymes for the tricarboxylic acid cycle or respiratory chain. Various saccharolytic enzymes were found, but many enzymes for amino acid biosynthesis were lacking in the genome. Twenty genes were newly identified as putative virulence factors of C. perfringens, and we found a total of five hyaluronidase genes that will also contribute to virulence. The genome analysis also proved an efficient method for finding four members of the two-component VirR/VirS regulon that coordinately regulates the pathogenicity of C. perfringens. Clearly, C perfringens obtains various essential materials from the host by producing several degradative enzymes and toxins, resulting in massive destruction of the host tissues.

Original languageEnglish
Pages (from-to)996-1001
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number2
DOIs
Publication statusPublished - 2002 Jan 22
Externally publishedYes

Fingerprint

Clostridium perfringens
Genome
Enzymes
Virulence
Enterotoxemia
Gas Gangrene
Regulon
Hyaluronoglucosaminidase
Citric Acid Cycle
Base Composition
Virulence Factors
Electron Transport
Spores
rRNA Genes
Open Reading Frames
Genes
Fermentation
Gases
Bacteria
Amino Acids

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. / Shimizu, Tohru; Ohtani, Kaori; Hirakawa, Hideki; Ohshima, Kenshiro; Yamashita, Atsushi; Shiba, Tadayoshi; Ogasawara, Naotake; Hattori, Masahira; Kuhara, Satoru; Hayashi, Hideo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 2, 22.01.2002, p. 996-1001.

Research output: Contribution to journalArticle

Shimizu, T, Ohtani, K, Hirakawa, H, Ohshima, K, Yamashita, A, Shiba, T, Ogasawara, N, Hattori, M, Kuhara, S & Hayashi, H 2002, 'Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 2, pp. 996-1001. https://doi.org/10.1073/pnas.022493799
Shimizu, Tohru ; Ohtani, Kaori ; Hirakawa, Hideki ; Ohshima, Kenshiro ; Yamashita, Atsushi ; Shiba, Tadayoshi ; Ogasawara, Naotake ; Hattori, Masahira ; Kuhara, Satoru ; Hayashi, Hideo. / Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 2. pp. 996-1001.
@article{7e2432ad7ae04a99bea729069edcb075,
title = "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater",
abstract = "Clostridium perfringens is a Gram-positive anaerobic spore-forming bacterium that causes life-threatening gas gangrene and mild enterotoxaemia in humans, although it colonizes as normal intestinal flora of humans and animals. The organism is known to produce a variety of toxins and enzymes that are responsible for the severe myonecrotic lesions. Here we report the complete 3,031,430-bp sequence of C. perfringens strain 13 that comprises 2,660 protein coding regions and 10 rRNA genes, showing pronounced low overall G + C content (28.6{\%}). The genome contains typical anaerobic fermentation enzymes leading to gas production but no enzymes for the tricarboxylic acid cycle or respiratory chain. Various saccharolytic enzymes were found, but many enzymes for amino acid biosynthesis were lacking in the genome. Twenty genes were newly identified as putative virulence factors of C. perfringens, and we found a total of five hyaluronidase genes that will also contribute to virulence. The genome analysis also proved an efficient method for finding four members of the two-component VirR/VirS regulon that coordinately regulates the pathogenicity of C. perfringens. Clearly, C perfringens obtains various essential materials from the host by producing several degradative enzymes and toxins, resulting in massive destruction of the host tissues.",
author = "Tohru Shimizu and Kaori Ohtani and Hideki Hirakawa and Kenshiro Ohshima and Atsushi Yamashita and Tadayoshi Shiba and Naotake Ogasawara and Masahira Hattori and Satoru Kuhara and Hideo Hayashi",
year = "2002",
month = "1",
day = "22",
doi = "10.1073/pnas.022493799",
language = "English",
volume = "99",
pages = "996--1001",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "2",

}

TY - JOUR

T1 - Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater

AU - Shimizu, Tohru

AU - Ohtani, Kaori

AU - Hirakawa, Hideki

AU - Ohshima, Kenshiro

AU - Yamashita, Atsushi

AU - Shiba, Tadayoshi

AU - Ogasawara, Naotake

AU - Hattori, Masahira

AU - Kuhara, Satoru

AU - Hayashi, Hideo

PY - 2002/1/22

Y1 - 2002/1/22

N2 - Clostridium perfringens is a Gram-positive anaerobic spore-forming bacterium that causes life-threatening gas gangrene and mild enterotoxaemia in humans, although it colonizes as normal intestinal flora of humans and animals. The organism is known to produce a variety of toxins and enzymes that are responsible for the severe myonecrotic lesions. Here we report the complete 3,031,430-bp sequence of C. perfringens strain 13 that comprises 2,660 protein coding regions and 10 rRNA genes, showing pronounced low overall G + C content (28.6%). The genome contains typical anaerobic fermentation enzymes leading to gas production but no enzymes for the tricarboxylic acid cycle or respiratory chain. Various saccharolytic enzymes were found, but many enzymes for amino acid biosynthesis were lacking in the genome. Twenty genes were newly identified as putative virulence factors of C. perfringens, and we found a total of five hyaluronidase genes that will also contribute to virulence. The genome analysis also proved an efficient method for finding four members of the two-component VirR/VirS regulon that coordinately regulates the pathogenicity of C. perfringens. Clearly, C perfringens obtains various essential materials from the host by producing several degradative enzymes and toxins, resulting in massive destruction of the host tissues.

AB - Clostridium perfringens is a Gram-positive anaerobic spore-forming bacterium that causes life-threatening gas gangrene and mild enterotoxaemia in humans, although it colonizes as normal intestinal flora of humans and animals. The organism is known to produce a variety of toxins and enzymes that are responsible for the severe myonecrotic lesions. Here we report the complete 3,031,430-bp sequence of C. perfringens strain 13 that comprises 2,660 protein coding regions and 10 rRNA genes, showing pronounced low overall G + C content (28.6%). The genome contains typical anaerobic fermentation enzymes leading to gas production but no enzymes for the tricarboxylic acid cycle or respiratory chain. Various saccharolytic enzymes were found, but many enzymes for amino acid biosynthesis were lacking in the genome. Twenty genes were newly identified as putative virulence factors of C. perfringens, and we found a total of five hyaluronidase genes that will also contribute to virulence. The genome analysis also proved an efficient method for finding four members of the two-component VirR/VirS regulon that coordinately regulates the pathogenicity of C. perfringens. Clearly, C perfringens obtains various essential materials from the host by producing several degradative enzymes and toxins, resulting in massive destruction of the host tissues.

UR - http://www.scopus.com/inward/record.url?scp=0037154260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037154260&partnerID=8YFLogxK

U2 - 10.1073/pnas.022493799

DO - 10.1073/pnas.022493799

M3 - Article

C2 - 11792842

AN - SCOPUS:0037154260

VL - 99

SP - 996

EP - 1001

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 2

ER -