Complete genome sequence of enterohemorrhagic Eschelichia coli O157

H7 and genomic comparison with a laboratory strain K-12

Tetsuya Hayashi, Kozo Makino, Makoto Ohnishi, Ken Kurokawa, Kazuo Ishii, Katsushi Yokoyama, Chang Gyun Han, Eiichi Ohtsubo, Keisuke Nakayama, Takahiro Murata, Masashi Tanaka, Toru Tobe, Tetsuya Iida, Hideto Takami, Takeshi Honda, Chihiro Sasakawa, Naotake Ogasawara, Teruo Yasunaga, Satoru Kuhara, Tadayoshi Shiba & 2 others Masahira Hattori, Hideo Shinagawa

Research output: Contribution to journalArticle

916 Citations (Scopus)

Abstract

Escherichia coli O157:H7 is a major food-borne infectious pathogen that causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. Here we report the complete chromosome sequence of an O157:H7 strain isolated from the Sakai outbreak, and the results of genomic comparison with a benign laboratory strain, K-12 MG1655. The chromosome is 5.5 Mb in size, 859 Kb larger than that of K-12. We identified a 4.1-Mb sequence highly conserved between the two strains, which may represent the fundamental backbone of the E. coli chromosome. The remaining 1.4-Mb sequence comprises of O157:H7-specific sequences, most of which are horizontally transferred foreign DNAs. The predominant roles of bacteriophages in the emergence of O157:H7 is evident by the presence of 24 prophages and prophage-like elements that occupy more than half of the O157:H7-specific sequences. The O157:H7 chromosome encodes 1632 proteins and 20 tRNAs that are not present in K-12. Among these, at least 131 proteins are assumed to have virulence-related functions. Genome-wide codon usage analysis suggested that the O157:H7-specific tRNAs are involved in the efficient expression of the strain-specific genes. A complete set of the genes specific to O157:H7 presented here sheds new insight into the pathogenicity and the physiology of O157:H7, and will open a way to fully understand the molecular mechanisms underlying the O157:H7 infection.

Original languageEnglish
Pages (from-to)11-22
Number of pages12
JournalDNA Research
Volume8
Issue number1
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Chromosomes
Genome
Prophages
Transfer RNA
Virulence
Hemolytic-Uremic Syndrome
Escherichia coli O157
Conserved Sequence
Colitis
Codon
Bacteriophages
Genes
Disease Outbreaks
Diarrhea
Proteins
Escherichia coli
Food
DNA
Infection

Keywords

  • Bacterial pathogenicity
  • E. coli K-12
  • E. coli O157:H7
  • Evolution
  • Genome sequence

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Hayashi, T., Makino, K., Ohnishi, M., Kurokawa, K., Ishii, K., Yokoyama, K., ... Shinagawa, H. (2001). Complete genome sequence of enterohemorrhagic Eschelichia coli O157: H7 and genomic comparison with a laboratory strain K-12. DNA Research, 8(1), 11-22.

Complete genome sequence of enterohemorrhagic Eschelichia coli O157 : H7 and genomic comparison with a laboratory strain K-12. / Hayashi, Tetsuya; Makino, Kozo; Ohnishi, Makoto; Kurokawa, Ken; Ishii, Kazuo; Yokoyama, Katsushi; Han, Chang Gyun; Ohtsubo, Eiichi; Nakayama, Keisuke; Murata, Takahiro; Tanaka, Masashi; Tobe, Toru; Iida, Tetsuya; Takami, Hideto; Honda, Takeshi; Sasakawa, Chihiro; Ogasawara, Naotake; Yasunaga, Teruo; Kuhara, Satoru; Shiba, Tadayoshi; Hattori, Masahira; Shinagawa, Hideo.

In: DNA Research, Vol. 8, No. 1, 2001, p. 11-22.

Research output: Contribution to journalArticle

Hayashi, T, Makino, K, Ohnishi, M, Kurokawa, K, Ishii, K, Yokoyama, K, Han, CG, Ohtsubo, E, Nakayama, K, Murata, T, Tanaka, M, Tobe, T, Iida, T, Takami, H, Honda, T, Sasakawa, C, Ogasawara, N, Yasunaga, T, Kuhara, S, Shiba, T, Hattori, M & Shinagawa, H 2001, 'Complete genome sequence of enterohemorrhagic Eschelichia coli O157: H7 and genomic comparison with a laboratory strain K-12', DNA Research, vol. 8, no. 1, pp. 11-22.
Hayashi T, Makino K, Ohnishi M, Kurokawa K, Ishii K, Yokoyama K et al. Complete genome sequence of enterohemorrhagic Eschelichia coli O157: H7 and genomic comparison with a laboratory strain K-12. DNA Research. 2001;8(1):11-22.
Hayashi, Tetsuya ; Makino, Kozo ; Ohnishi, Makoto ; Kurokawa, Ken ; Ishii, Kazuo ; Yokoyama, Katsushi ; Han, Chang Gyun ; Ohtsubo, Eiichi ; Nakayama, Keisuke ; Murata, Takahiro ; Tanaka, Masashi ; Tobe, Toru ; Iida, Tetsuya ; Takami, Hideto ; Honda, Takeshi ; Sasakawa, Chihiro ; Ogasawara, Naotake ; Yasunaga, Teruo ; Kuhara, Satoru ; Shiba, Tadayoshi ; Hattori, Masahira ; Shinagawa, Hideo. / Complete genome sequence of enterohemorrhagic Eschelichia coli O157 : H7 and genomic comparison with a laboratory strain K-12. In: DNA Research. 2001 ; Vol. 8, No. 1. pp. 11-22.
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AU - Ishii, Kazuo

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AU - Murata, Takahiro

AU - Tanaka, Masashi

AU - Tobe, Toru

AU - Iida, Tetsuya

AU - Takami, Hideto

AU - Honda, Takeshi

AU - Sasakawa, Chihiro

AU - Ogasawara, Naotake

AU - Yasunaga, Teruo

AU - Kuhara, Satoru

AU - Shiba, Tadayoshi

AU - Hattori, Masahira

AU - Shinagawa, Hideo

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