Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1

Tadashi Matsunaga, Yoshiko Okamura, Yorikane Fukuda, Aris Tri Wahyudi, Yaeko Murase, Haruko Takeyama

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Magnetospirillum sp. strain AMB-1 is a Gram-negative α- proteobacterium that synthesizes nano-sized magnetites, referred to as magnetosomes, aligned intracellularly in a chain. The potential of this nano-sized material is growing and will be applicable to broad research areas. It has been expected that genome analysis would elucidate the mechanism of magnetosome formation by magnetic bacteria. Here we describe the genome of Magnetospirillum sp. AMB-1 wild type, which consists of a single circular chromosome of 4 967 148 bp. For identification of genes required for magnetosome formation, transposon rnutagenesis and determination of magnetosome membrane proteins were performed. Analysis of a non-magnetic transposon mutant library focused on three unknown genes from 2752 unknown genes and three genes from 205 signal transduction genes. Partial proteome analysis of the magnetosome membrane revealed that the membrane contains numerous oxidation/reduction proteins and a signal response regulator that may function in magnetotaxis. Thus, oxidation/reduction proteins and elaborate multidomain signaling proteins were analyzed. This comprehensive genome analysis will enable resolution of the mechanisms of magnetosome formation and provide a template to determine how magnetic bacteria maintain a species-specific, nano-sized, magnetic single domain and paramagnetic morphology.

Original languageEnglish
Pages (from-to)157-166
Number of pages10
JournalDNA Research
Volume12
Issue number3
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Magnetospirillum
Magnetosomes
Anaerobic Bacteria
Genome
Genes
Oxidation-Reduction
Ferrosoferric Oxide
Bacteria
Proteobacteria
Chromosomes, Human, Pair 4
Proteins
Membranes
Proteome
Signal Transduction
Membrane Proteins

Keywords

  • Biomineralization
  • Magnetosome
  • Magnetotactic bacteria

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1. / Matsunaga, Tadashi; Okamura, Yoshiko; Fukuda, Yorikane; Wahyudi, Aris Tri; Murase, Yaeko; Takeyama, Haruko.

In: DNA Research, Vol. 12, No. 3, 2005, p. 157-166.

Research output: Contribution to journalArticle

Matsunaga, Tadashi ; Okamura, Yoshiko ; Fukuda, Yorikane ; Wahyudi, Aris Tri ; Murase, Yaeko ; Takeyama, Haruko. / Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1. In: DNA Research. 2005 ; Vol. 12, No. 3. pp. 157-166.
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