Complete genome sequence of the mitochondrial DNA of the sparkling enope squid, Watasenia scintillans

Keiko Hayashi, Yuri L. Kawai, Kei Yura, Masa Aki Yoshida, Atsushi Ogura, Kenichiro Hata, Kazuhiko Nakabayashi, Kohji Okamura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The sparkling enope squid, Watasenia scintillans, is a deep-sea mollusk inhabiting the western part of the Pacific Ocean. It has the peculiar ability to illuminate its body without the involvement of other organisms. In this study, we extracted the brain DNA from a single squid female caught in the Japan Sea and determined the complete genome sequence of its mitochondrial DNA using the Illumina sequencing platform. The circular sequence is 20,089 bp in length. Using the next-generation sequencing data, we also estimated the mean copy number of mitochondria per cell in the brain to be 108 by comparing the depths of the read data in the nuclear and mitochondrial genomes. The haploid genome size was calculated to be 4.78 Gb. Six heteroplasmy sites were also identified, together with their allele frequencies, in this individual. Our methodology is shown to be useful in mitochondrion-related studies.

Original languageEnglish
Pages (from-to)1842-1843
Number of pages2
JournalMitochondrial DNA. Part A, DNA mapping, sequencing, and analysis
Volume27
Issue number3
DOIs
Publication statusPublished - 2016 May 1
Externally publishedYes

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Keywords

  • Cephalopod
  • genome size
  • mollusk
  • sparkling enope squid
  • Watasenia scintillans

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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