Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation

Tulika Prakash, Kenshiro Oshima, Hidetoshi Morita, Shinji Fukuda, Akemi Imaoka, Naveen Kumar, Vineet K. Sharma, Seok Won Kim, Mahoko Takahashi, Naruya Saitou, Todd D. Taylor, Hiroshi Ohno, Yoshinori Umesaki, Masahira Hattori

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Segmented filamentous bacteria (SFB) are noncultivable commensals inhabiting the gut of various vertebrate species and have been shown to induce Th17 cells in mice. We present the complete genome sequences of both rat and mouse SFB isolated from SFB-monocolonized hosts. The rat and mouse SFB genomes each harbor a single circular chromosome of 1.52 and 1.59 Mb encoding 1346 and 1420 protein-coding genes, respectively. The overall nucleotide identity between the two genomes is 86%, and the substitution rate was estimated to be similar to that of the free-living E. coli. SFB genomes encode typical genes for anaerobic fermentation and spore and flagella formation, but lack most of the amino acid biosynthesis enzymes, reminiscent of pathogenic Clostridia, exhibiting large dependency on the host. However, SFB lack most of the clostridial virulence-related genes. Comparative analysis with clostridial genomes suggested possible mechanisms for host responses and specific adaptations in the intestine.

Original languageEnglish
Pages (from-to)273-284
Number of pages12
JournalCell Host and Microbe
Volume10
Issue number3
DOIs
Publication statusPublished - 2011 Sep 15
Externally publishedYes

Fingerprint

Th17 Cells
Helper-Inducer T-Lymphocytes
Cell Differentiation
Genome
Bacteria
Flagella
Clostridium
Spores
Genes
Fermentation
Intestines
Virulence
Vertebrates
Nucleotides
Chromosomes
Escherichia coli
Amino Acids
Enzymes
Proteins

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation. / Prakash, Tulika; Oshima, Kenshiro; Morita, Hidetoshi; Fukuda, Shinji; Imaoka, Akemi; Kumar, Naveen; Sharma, Vineet K.; Kim, Seok Won; Takahashi, Mahoko; Saitou, Naruya; Taylor, Todd D.; Ohno, Hiroshi; Umesaki, Yoshinori; Hattori, Masahira.

In: Cell Host and Microbe, Vol. 10, No. 3, 15.09.2011, p. 273-284.

Research output: Contribution to journalArticle

Prakash, T, Oshima, K, Morita, H, Fukuda, S, Imaoka, A, Kumar, N, Sharma, VK, Kim, SW, Takahashi, M, Saitou, N, Taylor, TD, Ohno, H, Umesaki, Y & Hattori, M 2011, 'Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation', Cell Host and Microbe, vol. 10, no. 3, pp. 273-284. https://doi.org/10.1016/j.chom.2011.08.007
Prakash, Tulika ; Oshima, Kenshiro ; Morita, Hidetoshi ; Fukuda, Shinji ; Imaoka, Akemi ; Kumar, Naveen ; Sharma, Vineet K. ; Kim, Seok Won ; Takahashi, Mahoko ; Saitou, Naruya ; Taylor, Todd D. ; Ohno, Hiroshi ; Umesaki, Yoshinori ; Hattori, Masahira. / Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation. In: Cell Host and Microbe. 2011 ; Vol. 10, No. 3. pp. 273-284.
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