Complete nucleotide sequence of the Japanese encephalitis virus genome RNA

Hideo Sumiyoshi, Chisato Mori, Isao Fuke, Kouichi Morita, Satoshi Kuhara, Jun Kondou, Yo Kikuchi, Hiroshi Nagamatu, Akira Igarashi

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Abstract

The complete nucleotide sequence of the Japanese encephalitis virus (JEV) genome RNA was determined. The JEV genome contains 10,976 nucleotides and encodes a single long open reading frame (ORF) of 10,296 nucleotides corresponding to 3432 amino acid residues. This long polypeptide is thought to be cleaved into three structural proteins and several nonstructural proteins of the virus. The genetic location of the three structural proteins was determined by comparing the deduced amino acid sequence from the nucleotide sequence with the N-terminal amino acid sequences that were determined from the three purified structural proteins. The C-terminal region of the ORF may encode an RNA-dependent RNA polymerase which has significant sequence homology with those of other RNA viruses.

Original languageEnglish
Pages (from-to)497-510
Number of pages14
JournalVirology
Volume161
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

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ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Sumiyoshi, H., Mori, C., Fuke, I., Morita, K., Kuhara, S., Kondou, J., Kikuchi, Y., Nagamatu, H., & Igarashi, A. (1987). Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology, 161(2), 497-510. https://doi.org/10.1016/0042-6822(87)90144-9