Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157

H7 derived from the Sakai outbreak

Kozo Makino, Katsushi Yokoyama, Yoshino Kubota, Chikako H. Yutsudo, Sigenobu Kimura, Ken Kurokawa, Kazuo Ishii, Masahira Hattori, Ichiro Tatsuno, Hiroyuki Abe, Tetsuya Iida, Koichiro Yamamoto, Makoto Onishi, Tetsuya Hayashi, Teruo Yasunaga, Takeshi Honda, Chihiro Sasakawa, Hideo Shinagawa

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The enterohemorrhagic Escherichia coli (EHEC) 0157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 and stx2 genes. In the EHEC strains, as well as in other VT-producing E. coli strains, the toxins are encoded by lysogenic bacteriophages. The EHEC 0157:H7 strain RIMD 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined the complete nucleotide sequence of a prophage, VT2-Sakai, carrying the stx2A and stx2B genes on the chromosome, and presumed the putative functions of the encoded proteins and the cis-acting DNA elements based on sequence homology data. To our surprise, the sequences in the regions of VT2-Sakai corresponding to the early gene regulators and replication proteins, and the DNA sequences recognized by the regulators share very limited homology to those of the VT2-encoding 933W phage carried by the EHEC 0157:H7 strain EDL933 reported by Plunkett et al. (J. Bacteriol., p1767-1778, 181, 1999), although the sequences corresponding to the structural components are almost identical. These data suggest that these two phages were derived from a common ancestral phage and that either or both of them underwent multiple genetic rearrangements. An IS629 insertion was found downstream of the stx2B gene and upstream of the lysis gene S, and this might be responsible for the absence of plaque-forming activity in the lysate obtained after inducing treatments.

Original languageEnglish
Pages (from-to)227-239
Number of pages13
JournalGenes and Genetic Systems
Volume74
Issue number5
DOIs
Publication statusPublished - 1999 Oct
Externally publishedYes

Fingerprint

Shiga Toxin 2
Shiga-like toxin 2
Enterohemorrhagic Escherichia coli
Prophages
enterohemorrhagic Escherichia coli
Bacteriophages
Escherichia coli O157
bacteriophages
Escherichia coli
Disease Outbreaks
Nucleotides
Genes
nucleotide sequences
genes
Shiga Toxins
verotoxins
DNA sequences
Regulator Genes
Chromosomes
Sequence Homology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Genetics(clinical)

Cite this

Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157 : H7 derived from the Sakai outbreak. / Makino, Kozo; Yokoyama, Katsushi; Kubota, Yoshino; Yutsudo, Chikako H.; Kimura, Sigenobu; Kurokawa, Ken; Ishii, Kazuo; Hattori, Masahira; Tatsuno, Ichiro; Abe, Hiroyuki; Iida, Tetsuya; Yamamoto, Koichiro; Onishi, Makoto; Hayashi, Tetsuya; Yasunaga, Teruo; Honda, Takeshi; Sasakawa, Chihiro; Shinagawa, Hideo.

In: Genes and Genetic Systems, Vol. 74, No. 5, 10.1999, p. 227-239.

Research output: Contribution to journalArticle

Makino, K, Yokoyama, K, Kubota, Y, Yutsudo, CH, Kimura, S, Kurokawa, K, Ishii, K, Hattori, M, Tatsuno, I, Abe, H, Iida, T, Yamamoto, K, Onishi, M, Hayashi, T, Yasunaga, T, Honda, T, Sasakawa, C & Shinagawa, H 1999, 'Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157: H7 derived from the Sakai outbreak', Genes and Genetic Systems, vol. 74, no. 5, pp. 227-239. https://doi.org/10.1266/ggs.74.227
Makino, Kozo ; Yokoyama, Katsushi ; Kubota, Yoshino ; Yutsudo, Chikako H. ; Kimura, Sigenobu ; Kurokawa, Ken ; Ishii, Kazuo ; Hattori, Masahira ; Tatsuno, Ichiro ; Abe, Hiroyuki ; Iida, Tetsuya ; Yamamoto, Koichiro ; Onishi, Makoto ; Hayashi, Tetsuya ; Yasunaga, Teruo ; Honda, Takeshi ; Sasakawa, Chihiro ; Shinagawa, Hideo. / Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157 : H7 derived from the Sakai outbreak. In: Genes and Genetic Systems. 1999 ; Vol. 74, No. 5. pp. 227-239.
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abstract = "The enterohemorrhagic Escherichia coli (EHEC) 0157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 and stx2 genes. In the EHEC strains, as well as in other VT-producing E. coli strains, the toxins are encoded by lysogenic bacteriophages. The EHEC 0157:H7 strain RIMD 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined the complete nucleotide sequence of a prophage, VT2-Sakai, carrying the stx2A and stx2B genes on the chromosome, and presumed the putative functions of the encoded proteins and the cis-acting DNA elements based on sequence homology data. To our surprise, the sequences in the regions of VT2-Sakai corresponding to the early gene regulators and replication proteins, and the DNA sequences recognized by the regulators share very limited homology to those of the VT2-encoding 933W phage carried by the EHEC 0157:H7 strain EDL933 reported by Plunkett et al. (J. Bacteriol., p1767-1778, 181, 1999), although the sequences corresponding to the structural components are almost identical. These data suggest that these two phages were derived from a common ancestral phage and that either or both of them underwent multiple genetic rearrangements. An IS629 insertion was found downstream of the stx2B gene and upstream of the lysis gene S, and this might be responsible for the absence of plaque-forming activity in the lysate obtained after inducing treatments.",
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AU - Yokoyama, Katsushi

AU - Kubota, Yoshino

AU - Yutsudo, Chikako H.

AU - Kimura, Sigenobu

AU - Kurokawa, Ken

AU - Ishii, Kazuo

AU - Hattori, Masahira

AU - Tatsuno, Ichiro

AU - Abe, Hiroyuki

AU - Iida, Tetsuya

AU - Yamamoto, Koichiro

AU - Onishi, Makoto

AU - Hayashi, Tetsuya

AU - Yasunaga, Teruo

AU - Honda, Takeshi

AU - Sasakawa, Chihiro

AU - Shinagawa, Hideo

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