Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids

Hirokazu Yano, Masatoshi Miyakoshi, Kenshiro Ohshima, Michiro Tabata, Yuji Nagata, Masahira Hattori, Masataka Tsuda

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Abstract

To understand the mechanisms for structural diversification of Pseudomonas-derived toluene-catabolic (TOL) plasmids, the complete sequence of a self-transmissible plasmid pDK1 with a size of 128,921 bp from Pseudomonas putida HS1 was determined. Comparative analysis revealed that (i) pDK1 consisted of a 75.6-kb IncP-7 plasmid backbone and 53.2-kb accessory gene segments that were bounded by transposon-associated regions, (ii) the genes for conjugative transfer of pDK1 were highly similar to those of MOBH group of mobilizable plasmids, and (iii) the toluene-catabolic (xyl) gene clusters of pDK1 were derived through homologous recombination, transposition, and site-specific recombination from the xyl gene clusters homologous to another TOL plasmid, pWW53. The minireplicons of pDK1 and its related IncP-7 plasmids, pWW53 and pCAR1, that contain replication and partition genes were maintained in all of six Pseudomonas strains tested, but not in alpha- or betaproteobacterial strains. The recipient host range of conjugative transfer of pDK1 was, however, limited to two Pseudomonas strains. These results indicate that IncP-7 plasmids are essentially narrow-host-range and self-transmissible plasmids that encode MOBH group-related transfer functions and that the host range of IncP-7-specified conjugative transfer was, unlike the situation in other well-known plasmids, narrower than that of its replication.

Original languageEnglish
Pages (from-to)4337-4347
Number of pages11
JournalJournal of Bacteriology
Volume192
Issue number17
DOIs
Publication statusPublished - 2010 Sep
Externally publishedYes

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Toluene
Plasmids
Host Specificity
Pseudomonas
Multigene Family
Genes
Pseudomonas putida
Homologous Recombination
Genetic Recombination

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids. / Yano, Hirokazu; Miyakoshi, Masatoshi; Ohshima, Kenshiro; Tabata, Michiro; Nagata, Yuji; Hattori, Masahira; Tsuda, Masataka.

In: Journal of Bacteriology, Vol. 192, No. 17, 09.2010, p. 4337-4347.

Research output: Contribution to journalArticle

Yano, Hirokazu ; Miyakoshi, Masatoshi ; Ohshima, Kenshiro ; Tabata, Michiro ; Nagata, Yuji ; Hattori, Masahira ; Tsuda, Masataka. / Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids. In: Journal of Bacteriology. 2010 ; Vol. 192, No. 17. pp. 4337-4347.
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