Comprehensive evaluation of leukocyte lineage derived from human hematopoietic cells in humanized mice

Masayuki Takahashi, Noriyuki Tsujimura, Kensuke Otsuka, Tomoko Yoshino, Tetsushi Mori, Tadashi Matsunaga, Satoshi Nakasono

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recently, humanized animals whereby a part of the animal is biologically engineered using human genes or cells have been utilized to overcome interspecific differences. Herein, we analyzed the detail of the differentiation states of various human leukocyte subpopulations in humanized mouse and evaluated comprehensively the similarity of the leukocyte lineage between humanized mice and humans. Humanized mice were established by transplanting human CD34+ cord blood cells into irradiated severely immunodeficient NOD/Shi-scid/IL2Rγnull (NOG) mice, and the phenotypes of human cells contained in bone marrow, thymus, spleen and peripheral blood from the mice were analyzed at monthly intervals until 4months after cell transplantation. The analysis revealed that transplanted human hematopoietic stem cells via the caudal vein homed and engrafted themselves successfully at the mouse bone marrow. Subsequently, the differentiated leukocytes migrated to the various tissues. Almost all of the leukocytes within the thymus were human cells. Furthermore, analysis of the differentiation states of human leukocytes in various tissues and organs indicated that it is highly likely that the human-like leukocyte lineage can be developed in mice.

Original languageEnglish
Pages (from-to)529-535
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number4
DOIs
Publication statusPublished - 2012 Apr
Externally publishedYes

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Keywords

  • Cell transplantation
  • Hematopoietic stem cell
  • Humanized mice
  • Leukocyte lineage
  • NOG mice

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Takahashi, M., Tsujimura, N., Otsuka, K., Yoshino, T., Mori, T., Matsunaga, T., & Nakasono, S. (2012). Comprehensive evaluation of leukocyte lineage derived from human hematopoietic cells in humanized mice. Journal of Bioscience and Bioengineering, 113(4), 529-535. https://doi.org/10.1016/j.jbiosc.2011.11.011