Comprehensive reduction of amino acid set in a protein suggests the importance of prebiotic amino acids for stable proteins

Rei Shibue, Takahiro Sasamoto, Masami Shimada, Bowen Zhang, Akihiko Yamagishi, Satoshi Akanuma

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Modern organisms commonly use the same set of 20 genetically coded amino acids for protein synthesis with very few exceptions. However, earlier protein synthesis was plausibly much simpler than modern one and utilized only a limited set of amino acids. Nevertheless, few experimental tests of this issue with arbitrarily chosen amino acid sets had been reported prior to this report. Herein we comprehensively and systematically reduced the size of the amino acid set constituting an ancestral nucleoside kinase that was reconstructed in our previous study. We eventually found that two convergent sequences, each comprised of a 13-amino acid alphabet, folded into soluble, stable and catalytically active structures, even though their stabilities and activities were not as high as those of the parent protein. Notably, many but not all of the reduced-set amino acids coincide with those plausibly abundant in primitive Earth. The inconsistent amino acids appeared to be important for catalytic activity but not for stability. Therefore, our findings suggest that the prebiotically abundant amino acids were used for creating stable protein structures and other amino acids with functional side chains were recruited to achieve efficient catalysis.

    Original languageEnglish
    Article number1227
    JournalScientific Reports
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 2018 Dec 1

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    Prebiotics
    Amino Acids
    Proteins
    nucleoside phosphotransferase
    Catalysis

    ASJC Scopus subject areas

    • General

    Cite this

    Comprehensive reduction of amino acid set in a protein suggests the importance of prebiotic amino acids for stable proteins. / Shibue, Rei; Sasamoto, Takahiro; Shimada, Masami; Zhang, Bowen; Yamagishi, Akihiko; Akanuma, Satoshi.

    In: Scientific Reports, Vol. 8, No. 1, 1227, 01.12.2018.

    Research output: Contribution to journalArticle

    Shibue, Rei ; Sasamoto, Takahiro ; Shimada, Masami ; Zhang, Bowen ; Yamagishi, Akihiko ; Akanuma, Satoshi. / Comprehensive reduction of amino acid set in a protein suggests the importance of prebiotic amino acids for stable proteins. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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