Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry

Nanako Azegami, Kazumi Saikusa, Yasuto Todokoro, Aritaka Nagadoi, Hitoshi Kurumizaka, Yoshifumi Nishimura, Satoko Akashi

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.

    Original languageEnglish
    Pages (from-to)5155-5157
    Number of pages3
    JournalBiochemistry
    Volume52
    Issue number31
    DOIs
    Publication statusPublished - 2013 Aug 6

    Fingerprint

    Nucleosomes
    Mass spectrometry
    Mass Spectrometry
    Native Polyacrylamide Gel Electrophoresis
    Electrospray ionization
    Electrospray Ionization Mass Spectrometry
    Electrophoresis
    Dimers

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Azegami, N., Saikusa, K., Todokoro, Y., Nagadoi, A., Kurumizaka, H., Nishimura, Y., & Akashi, S. (2013). Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry. Biochemistry, 52(31), 5155-5157. https://doi.org/10.1021/bi4005655

    Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry. / Azegami, Nanako; Saikusa, Kazumi; Todokoro, Yasuto; Nagadoi, Aritaka; Kurumizaka, Hitoshi; Nishimura, Yoshifumi; Akashi, Satoko.

    In: Biochemistry, Vol. 52, No. 31, 06.08.2013, p. 5155-5157.

    Research output: Contribution to journalArticle

    Azegami, N, Saikusa, K, Todokoro, Y, Nagadoi, A, Kurumizaka, H, Nishimura, Y & Akashi, S 2013, 'Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry', Biochemistry, vol. 52, no. 31, pp. 5155-5157. https://doi.org/10.1021/bi4005655
    Azegami N, Saikusa K, Todokoro Y, Nagadoi A, Kurumizaka H, Nishimura Y et al. Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry. Biochemistry. 2013 Aug 6;52(31):5155-5157. https://doi.org/10.1021/bi4005655
    Azegami, Nanako ; Saikusa, Kazumi ; Todokoro, Yasuto ; Nagadoi, Aritaka ; Kurumizaka, Hitoshi ; Nishimura, Yoshifumi ; Akashi, Satoko. / Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry. In: Biochemistry. 2013 ; Vol. 52, No. 31. pp. 5155-5157.
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