Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast

Yasutaka Kakui, Adam Rabinowitz, David J. Barry, Frank Uhlmann

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The eukaryotic genome consists of DNA molecules far longer than the cells that contain them. They reach their greatest compaction during chromosome condensation in mitosis. This process is aided by condensin, a structural maintenance of chromosomes (SMC) family member1,2. The spatial organization of mitotic chromosomes and how condensin shapes chromatin architecture are not yet fully understood. Here we use chromosome conformation capture (Hi-C)3,4 to study mitotic chromosome condensation in the fission yeast Schizosaccharomyces pombe5-7. This showed that the interphase landscape characterized by small chromatin domains is replaced by fewer but larger domains in mitosis. Condensin achieves this by setting up longer-range, intrachromosomal DNA interactions, which compact and individualize chromosomes. At the same time, local chromatin contacts are constrained by condensin, with profound implications for local chromatin function during mitosis. Our results highlight condensin as a major determinant that changes the chromatin landscape as cells prepare their genomes for cell division.

Original languageEnglish
Pages (from-to)1553-1557
Number of pages5
JournalNature Genetics
Volume49
Issue number10
DOIs
Publication statusPublished - 2017 Oct 1
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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