Conformational changes at the highly reactive cystein and lysine regions of skeletal muscle myosin induced by formation of transition state analogues

Shinsaku Maruta, Kazuaki Homma, Takashi Ohki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Myosin forms stable ternary complexes with Mg2+-ADP and phosphate analogues of aluminum fluoride (AlF4 -), beryllium fluoride (BeFn), and scandium fluoride (ScFn). These complexes are distinct from each other and may mimic different transient states in the ATPase cycle. Regions of skeletal muscle myosin containing the highly reactive residues Cys 707 (SH1), Cys 697 (SH2), and lysine 83 (RLR) dramatically alter their local conformation when myosin hydrolyzes ATP, and these changes may reflect formation of a series of transient intermediates during ATP hydrolysis. We used the fluorescent probes 4-fluoro-7-sulfamoylbezofurazan, 2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid, and trinitrobenzene-sulfonate, which bind to SH1, SH2, and RLR, respectively, to examine differences in local conformations within myosin·ADP·phosphate analogue (BeFn, V(i), AlF4 -, and ScFn) complexes. It was observed that the ternary complexes had SH1 conformations similar to those seen on S-1 in the presence of ATP. In contrast, local conformations in the SH2 and RLR regions of S-1·ADP·BeFn were different from those in corresponding regions of S-1·ADP·AlF4 - or ScFn. These results suggest that SH1 and SH2 move distinctly during ATP hydrolysis and that the local conformations of the SH2 and RLR regions more sensitively reflect different transient states.

Original languageEnglish
Pages (from-to)578-584
Number of pages7
JournalJournal of Biochemistry
Volume124
Issue number3
Publication statusPublished - 1998 Sep
Externally publishedYes

Fingerprint

Skeletal Muscle Myosins
Lysine
Conformations
Adenosine Triphosphate
Myosins
Hydrolysis
Scandium
Trinitrobenzenesulfonic Acid
Fluorides
Fluorescent Dyes
Adenosine Diphosphate
Adenosine Triphosphatases
Phosphates

Keywords

  • ATP analogues
  • Chemical modification
  • Energy transduction
  • Fluorescent probes
  • Myosin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Conformational changes at the highly reactive cystein and lysine regions of skeletal muscle myosin induced by formation of transition state analogues. / Maruta, Shinsaku; Homma, Kazuaki; Ohki, Takashi.

In: Journal of Biochemistry, Vol. 124, No. 3, 09.1998, p. 578-584.

Research output: Contribution to journalArticle

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